Investigation of poloxamer cell protective ability via shear sensitive aggregates in stirred aerated bioreactor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F22%3A43925033" target="_blank" >RIV/60461373:22340/22:43925033 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1369703X22002182?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1369703X22002182?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bej.2022.108549" target="_blank" >10.1016/j.bej.2022.108549</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Investigation of poloxamer cell protective ability via shear sensitive aggregates in stirred aerated bioreactor

Popis výsledku v původním jazyce

Mammalian cell cultivation in bioreactors is widely practiced in the pharmaceutical industry for production of monoclonal antibodies, vaccines and other therapeutic products. During cell culture, ensuring homogeneity and oxygen transport to the cultivation broth is essential for survival and proper cell growth. Due to the lack of a cell wall, mammalian cells are prone to damage by the gas aeration. Common practice how to protect mammalian cells against hydrodynamic forces is usage of poloxamers – synthetic, water-soluble nonionic copolymers. However, poloxamers are never pure compounds and the distribution of molecular weight strongly depends on the production process. This may lead to the final product containing impurities, which may have a negative impact on the protectivity of cells. Therefore, it is of high importance to know whether a particular poloxamer can be used as a cell protective agent. In this paper, we extend the usability of our method based on shear sensitive poly(methyl methacrylate) (PMMA) aggregate probes to investigate the protective capability of poloxamers. Comparative study of three different poloxamers and their impact on maximum effective hydrodynamic stress (τmax) and mass transfer coefficient (kLa) is conducted in the bench-scale 3.5 L Minifors bioreactor.

Název v anglickém jazyce

Investigation of poloxamer cell protective ability via shear sensitive aggregates in stirred aerated bioreactor

Popis výsledku anglicky

Mammalian cell cultivation in bioreactors is widely practiced in the pharmaceutical industry for production of monoclonal antibodies, vaccines and other therapeutic products. During cell culture, ensuring homogeneity and oxygen transport to the cultivation broth is essential for survival and proper cell growth. Due to the lack of a cell wall, mammalian cells are prone to damage by the gas aeration. Common practice how to protect mammalian cells against hydrodynamic forces is usage of poloxamers – synthetic, water-soluble nonionic copolymers. However, poloxamers are never pure compounds and the distribution of molecular weight strongly depends on the production process. This may lead to the final product containing impurities, which may have a negative impact on the protectivity of cells. Therefore, it is of high importance to know whether a particular poloxamer can be used as a cell protective agent. In this paper, we extend the usability of our method based on shear sensitive poly(methyl methacrylate) (PMMA) aggregate probes to investigate the protective capability of poloxamers. Comparative study of three different poloxamers and their impact on maximum effective hydrodynamic stress (τmax) and mass transfer coefficient (kLa) is conducted in the bench-scale 3.5 L Minifors bioreactor.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Engineering Journal

ISSN

1369-703X

e-ISSN

1873-295X

Svazek periodika

186

Číslo periodika v rámci svazku

AUG 2022

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

7

Strana od-do

108549

Kód UT WoS článku

000891992900007

EID výsledku v databázi Scopus

2-s2.0-85135357154