Application of matrix-assisted laser desorption/ionization mass spectrometry imaging in combination with LC-MS in pharmacokinetic study of metformin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F18%3A43913595" target="_blank" >RIV/60461373:22340/18:43913595 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/18:00489365

Výsledek na webu

<a href="https://www.future-science.com/doi/full/10.4155/bio-2017-0190" target="_blank" >https://www.future-science.com/doi/full/10.4155/bio-2017-0190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4155/bio-2017-0190" target="_blank" >10.4155/bio-2017-0190</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of matrix-assisted laser desorption/ionization mass spectrometry imaging in combination with LC-MS in pharmacokinetic study of metformin

Popis výsledku v původním jazyce

To demonstrate and discuss the pros and cons of various conventional and innovative analytical approaches. Methodology &amp; results: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI) of tissue sections as well as the extraction of tissue homogenates, blood plasma and dried blood spots coupled with LC-MS were employed to monitor the pharmacokinetics of metformin in mice. The time profile of metformin measured by matrix-assisted laser desorption/ionization MSI correlated well with the results found by LC-MS. Repeatability of the preparation of tissue sections for MSI was very good. Conclusion: MSI provided valuable information on the spatial distribution and relative concentration of the analyte within tissue sections. The analysis of the extracts of tissue homogenates, blood plasma and blood spots provided quantitative data on metformin. The dried blood spot approach is a progressive method of sampling, especially in studies where the amount of available blood is limited.

Název v anglickém jazyce

Application of matrix-assisted laser desorption/ionization mass spectrometry imaging in combination with LC-MS in pharmacokinetic study of metformin

Popis výsledku anglicky

To demonstrate and discuss the pros and cons of various conventional and innovative analytical approaches. Methodology &amp; results: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI) of tissue sections as well as the extraction of tissue homogenates, blood plasma and dried blood spots coupled with LC-MS were employed to monitor the pharmacokinetics of metformin in mice. The time profile of metformin measured by matrix-assisted laser desorption/ionization MSI correlated well with the results found by LC-MS. Repeatability of the preparation of tissue sections for MSI was very good. Conclusion: MSI provided valuable information on the spatial distribution and relative concentration of the analyte within tissue sections. The analysis of the extracts of tissue homogenates, blood plasma and blood spots provided quantitative data on metformin. The dried blood spot approach is a progressive method of sampling, especially in studies where the amount of available blood is limited.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LO1601" target="_blank" >LO1601: Pražské vysokoškolské analytické centrum II a III - NPU 2015-2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioanalysis

ISSN

1757-6180

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

71-81

Kód UT WoS článku

000419158500003

EID výsledku v databázi Scopus

2-s2.0-85040198311