Improving QCM Biosensor Efficiency by Flow Cell Modification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F25%3A43933489" target="_blank" >RIV/60461373:22340/25:43933489 - isvavai.cz</a>

Výsledek na webu

<a href="http://www2.it.cas.cz/fm/im/im/proceeding/2025/21" target="_blank" >http://www2.it.cas.cz/fm/im/im/proceeding/2025/21</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14311/TPFM.2025.021" target="_blank" >10.14311/TPFM.2025.021</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Improving QCM Biosensor Efficiency by Flow Cell Modification

Popis výsledku v původním jazyce

Biosensors based on quartz crystal microbalance (QCM) are often used for detection of biomolecules, pathogens, or viruses. The biological analyte gets captured at the QCM crystal surface, leading to a change in the crystal resonant frequency and analyte detection. However, with standard biosensor geometry, most of the analyte is captured in regions where the crystal sensitivity is limited. In this work, we investigate how changes in the biosensor geometry influence the spatial distribution of the captured analyte. Using a custom implementation of an immersed boundary method, we add obstacles on the non-detecting top wall of the biosensor flow cell, which is motivated by the possibility to 3D print this surface. Next, we simulate the biomolecules as Lagrangian particles with the Brownian motion taken into account and evaluate the probability of the analyte detection. Several different variants of obstacle shapes and layouts were tested. With the best obstacle configuration found, the amount of analyte captured in the most sensitive region of the crystal was 17% higher than in the standard configuration. The obtained results may be used (i) to generate QCM sensor geometries and validate this computational study, and (ii) as a basis for the development of less computationally demanding models usable in automatic optimization of the QCM biosensor geometry.

Název v anglickém jazyce

Improving QCM Biosensor Efficiency by Flow Cell Modification

Popis výsledku anglicky

Biosensors based on quartz crystal microbalance (QCM) are often used for detection of biomolecules, pathogens, or viruses. The biological analyte gets captured at the QCM crystal surface, leading to a change in the crystal resonant frequency and analyte detection. However, with standard biosensor geometry, most of the analyte is captured in regions where the crystal sensitivity is limited. In this work, we investigate how changes in the biosensor geometry influence the spatial distribution of the captured analyte. Using a custom implementation of an immersed boundary method, we add obstacles on the non-detecting top wall of the biosensor flow cell, which is motivated by the possibility to 3D print this surface. Next, we simulate the biomolecules as Lagrangian particles with the Brownian motion taken into account and evaluate the probability of the analyte detection. Several different variants of obstacle shapes and layouts were tested. With the best obstacle configuration found, the amount of analyte captured in the most sensitive region of the crystal was 17% higher than in the standard configuration. The obtained results may be used (i) to generate QCM sensor geometries and validate this computational study, and (ii) as a basis for the development of less computationally demanding models usable in automatic optimization of the QCM biosensor geometry.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10102 - Applied mathematics

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Topical Problems of Fluid Mechanics 2025

ISBN

978-80-87012-05-5

ISSN

2336-5781

e-ISSN

—

Počet stran výsledku

8

Strana od-do

159-166

Název nakladatele

Ústav termomechaniky AV ČR, v.v.i.

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

12. 2. 2025

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

001597458700021