Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F16%3A00467340" target="_blank" >RIV/61388955:_____/16:00467340 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/16:10336936

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elan.201600273" target="_blank" >http://dx.doi.org/10.1002/elan.201600273</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201600273" target="_blank" >10.1002/elan.201600273</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

Popis výsledku v původním jazyce

Two enzymatic biosensors with amalgam powder reactors and twelve enzymatic biosensors with various silica powder reactors were fabricated and tested in this work. The enzymatic reactors based on silver amalgam powder provide high sensitivity and they are convenient for faster flow rates. Experiments with six silica materials showed that mesoporous silica SBA-15 was the best one in terms of covering by enzymes, sensitivity and lifetime of biosensors. The current response of the SBA15-glucose oxidase (GOx) sensor started to decrease only after 200 day testing and after 406 days the peak current was still 35.9 % of the initial value. The used amalgam tubular detector in a flow system allowed working at highly negative potentials, which significantly increased the sensitivity of determinations. Statistical results of parallel measurement of model solutions with the fabricated biosensors show their high accuracy (RSD=0.28–1.81 %) and sensitivity (6.2–14.3 µmol L1). The proposed SBA15-GOx biosensor was successfully used for determination of glucose in commercial honey.

Název v anglickém jazyce

Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

Popis výsledku anglicky

Two enzymatic biosensors with amalgam powder reactors and twelve enzymatic biosensors with various silica powder reactors were fabricated and tested in this work. The enzymatic reactors based on silver amalgam powder provide high sensitivity and they are convenient for faster flow rates. Experiments with six silica materials showed that mesoporous silica SBA-15 was the best one in terms of covering by enzymes, sensitivity and lifetime of biosensors. The current response of the SBA15-glucose oxidase (GOx) sensor started to decrease only after 200 day testing and after 406 days the peak current was still 35.9 % of the initial value. The used amalgam tubular detector in a flow system allowed working at highly negative potentials, which significantly increased the sensitivity of determinations. Statistical results of parallel measurement of model solutions with the fabricated biosensors show their high accuracy (RSD=0.28–1.81 %) and sensitivity (6.2–14.3 µmol L1). The proposed SBA15-GOx biosensor was successfully used for determination of glucose in commercial honey.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA15-03139S" target="_blank" >GA15-03139S: Nové elektroforetické přístupy pro studium obezity a diabetu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Svazek periodika

28

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

3028-3038

Kód UT WoS článku

000392824200017

EID výsledku v databázi Scopus

2-s2.0-85003422157