Different Mechanisms of DNA Radiosensitization by 8-Bromoadenosine and 2 '-Deoxy-2 '-fluorocytidine Observed on DNA Origami Nanoframe Supports

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F22%3A00558117" target="_blank" >RIV/61388955:_____/22:00558117 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/22:00558117 RIV/61389005:_____/22:00558117

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acs.jpclett.2c00584" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jpclett.2c00584</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.jpclett.2c00584" target="_blank" >10.1021/acs.jpclett.2c00584</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Different Mechanisms of DNA Radiosensitization by 8-Bromoadenosine and 2 '-Deoxy-2 '-fluorocytidine Observed on DNA Origami Nanoframe Supports

Popis výsledku v původním jazyce

DNA origami nanoframes with two parallel DNA sequences are used to evaluate the effect of nucleoside substituents on radiation-induced DNA damage. Double strand breaks (DSB) of DNA are counted using atomic force microscopy (AFM), and total number of lesions is evaluated using real-time polymerase chain reaction (RT-PCR). Enhanced AT or GC content does not increase the number of DNA strand breaks. Incorporation of 8-bromoadenosine results in the highest enhancement in total number of lesions. However, the highest enhancement in DSB is observed for 2´-deoxy-2´-fluorocytidine, indicating different mechanisms of radiosensitization by nucleoside analogues with the halogen substituent on base or sugar moieties, respectively. ´´Bystander´´ effects are observed, when the number of DSB in a sequence is enhanced by a substituent in the parallel DNA sequence. The present approach eliminates limitations of previously developed methods and motivates detailed studies of poorly understood conformation or bystander effects in radiation induced damage to DNA.

Název v anglickém jazyce

Different Mechanisms of DNA Radiosensitization by 8-Bromoadenosine and 2 '-Deoxy-2 '-fluorocytidine Observed on DNA Origami Nanoframe Supports

Popis výsledku anglicky

DNA origami nanoframes with two parallel DNA sequences are used to evaluate the effect of nucleoside substituents on radiation-induced DNA damage. Double strand breaks (DSB) of DNA are counted using atomic force microscopy (AFM), and total number of lesions is evaluated using real-time polymerase chain reaction (RT-PCR). Enhanced AT or GC content does not increase the number of DNA strand breaks. Incorporation of 8-bromoadenosine results in the highest enhancement in total number of lesions. However, the highest enhancement in DSB is observed for 2´-deoxy-2´-fluorocytidine, indicating different mechanisms of radiosensitization by nucleoside analogues with the halogen substituent on base or sugar moieties, respectively. ´´Bystander´´ effects are observed, when the number of DSB in a sequence is enhanced by a substituent in the parallel DNA sequence. The present approach eliminates limitations of previously developed methods and motivates detailed studies of poorly understood conformation or bystander effects in radiation induced damage to DNA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physical Chemistry Letters

ISSN

1948-7185

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

3922-3928

Kód UT WoS článku

000799352500021

EID výsledku v databázi Scopus

2-s2.0-85129336954