High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00568897" target="_blank" >RIV/61388955:_____/23:00568897 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11310/23:10472886
Výsledek na webu
<a href="https://hdl.handle.net/11104/0340175" target="_blank" >https://hdl.handle.net/11104/0340175</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2023.142033" target="_blank" >10.1016/j.electacta.2023.142033</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode
Popis výsledku v původním jazyce
L-lactic acid (LA) is an essential compound in the winemaking process. Furthermore, it is considered a significant biomarker for the diagnosis of a wide range of diseases, including cancer. Therefore, a cost-effective and reliable biosensing system for FIA is developed here for rapid and selective determination of LA. The biosensor architecture includes an easily replaceable lactate oxidase (LOx)-based mini-reactor connected in front of a silver amalgam screen-printed electrode acting as a transducer. The mini-reactor represents a tube filled with mesoporous silica powder (SBA−15) coated with covalently immobilized LOx. The principle of LA determination is based on the amperometric monitoring of oxygen consumption by its four-electron reduction at −900 mV vs. Ag pseudo-reference electrode, which avoids the interferences from commonly interfering compounds. The application of a strategy of a spatially separated biorecognition and detection part allowed for the immobilization of a notably large amount of enzyme (one mini-reactor contains c.a. 270 μg of the LOx), which, in turn, ensured excellent operational (93.8% of the initial signal after 350 measurements) and storage (96.9% of the initial biosensor response after 7 months) stability. The newly developed high-performance biosensor was successfully tested for the quantification of LA in saliva, wine, and dairy products.
Název v anglickém jazyce
High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode
Popis výsledku anglicky
L-lactic acid (LA) is an essential compound in the winemaking process. Furthermore, it is considered a significant biomarker for the diagnosis of a wide range of diseases, including cancer. Therefore, a cost-effective and reliable biosensing system for FIA is developed here for rapid and selective determination of LA. The biosensor architecture includes an easily replaceable lactate oxidase (LOx)-based mini-reactor connected in front of a silver amalgam screen-printed electrode acting as a transducer. The mini-reactor represents a tube filled with mesoporous silica powder (SBA−15) coated with covalently immobilized LOx. The principle of LA determination is based on the amperometric monitoring of oxygen consumption by its four-electron reduction at −900 mV vs. Ag pseudo-reference electrode, which avoids the interferences from commonly interfering compounds. The application of a strategy of a spatially separated biorecognition and detection part allowed for the immobilization of a notably large amount of enzyme (one mini-reactor contains c.a. 270 μg of the LOx), which, in turn, ensured excellent operational (93.8% of the initial signal after 350 measurements) and storage (96.9% of the initial biosensor response after 7 months) stability. The newly developed high-performance biosensor was successfully tested for the quantification of LA in saliva, wine, and dairy products.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Návaznosti výsledku
Projekt
<a href="/cs/project/GA20-07350S" target="_blank" >GA20-07350S: Elektrochemické a analytické aspekty transportu návykových a psychotropních látek přes modelové biologické bariery</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electrochimica acta
ISSN
0013-4686
e-ISSN
1873-3859
Svazek periodika
445
Číslo periodika v rámci svazku
MAR 2023
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
11
Strana od-do
142033
Kód UT WoS článku
000990156800001
EID výsledku v databázi Scopus
2-s2.0-85148323718