High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode

Kód výsledku v IS VaVaI

RIV/61388955:_____/23:00568897 - isvavai.cz

Nalezeny alternativní kódy

RIV/00216208:11310/23:10472886

Výsledek na webu

https://hdl.handle.net/11104/0340175

DOI - Digital Object Identifier

10.1016/j.electacta.2023.142033

Jazyk výsledku

angličtina

Název v původním jazyce

High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode

Popis výsledku v původním jazyce

L-lactic acid (LA) is an essential compound in the winemaking process. Furthermore, it is considered a significant biomarker for the diagnosis of a wide range of diseases, including cancer. Therefore, a cost-effective and reliable biosensing system for FIA is developed here for rapid and selective determination of LA. The biosensor architecture includes an easily replaceable lactate oxidase (LOx)-based mini-reactor connected in front of a silver amalgam screen-printed electrode acting as a transducer. The mini-reactor represents a tube filled with mesoporous silica powder (SBA−15) coated with covalently immobilized LOx. The principle of LA determination is based on the amperometric monitoring of oxygen consumption by its four-electron reduction at −900 mV vs. Ag pseudo-reference electrode, which avoids the interferences from commonly interfering compounds. The application of a strategy of a spatially separated biorecognition and detection part allowed for the immobilization of a notably large amount of enzyme (one mini-reactor contains c.a. 270 μg of the LOx), which, in turn, ensured excellent operational (93.8% of the initial signal after 350 measurements) and storage (96.9% of the initial biosensor response after 7 months) stability. The newly developed high-performance biosensor was successfully tested for the quantification of LA in saliva, wine, and dairy products.

Název v anglickém jazyce

High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode

Popis výsledku anglicky

L-lactic acid (LA) is an essential compound in the winemaking process. Furthermore, it is considered a significant biomarker for the diagnosis of a wide range of diseases, including cancer. Therefore, a cost-effective and reliable biosensing system for FIA is developed here for rapid and selective determination of LA. The biosensor architecture includes an easily replaceable lactate oxidase (LOx)-based mini-reactor connected in front of a silver amalgam screen-printed electrode acting as a transducer. The mini-reactor represents a tube filled with mesoporous silica powder (SBA−15) coated with covalently immobilized LOx. The principle of LA determination is based on the amperometric monitoring of oxygen consumption by its four-electron reduction at −900 mV vs. Ag pseudo-reference electrode, which avoids the interferences from commonly interfering compounds. The application of a strategy of a spatially separated biorecognition and detection part allowed for the immobilization of a notably large amount of enzyme (one mini-reactor contains c.a. 270 μg of the LOx), which, in turn, ensured excellent operational (93.8% of the initial signal after 350 measurements) and storage (96.9% of the initial biosensor response after 7 months) stability. The newly developed high-performance biosensor was successfully tested for the quantification of LA in saliva, wine, and dairy products.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

GA20-07350S: Elektrochemické a analytické aspekty transportu návykových a psychotropních látek přes modelové biologické bariery

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

1873-3859

Svazek periodika

445

Číslo periodika v rámci svazku

MAR 2023

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

142033

Kód UT WoS článku

000990156800001

EID výsledku v databázi Scopus

2-s2.0-85148323718