Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F24%3A00583598" target="_blank" >RIV/61388955:_____/24:00583598 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/24:10487974

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1567539424000252?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1567539424000252?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioelechem.2024.108663" target="_blank" >10.1016/j.bioelechem.2024.108663</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes.

Popis výsledku v původním jazyce

A new type of electrochemical biosensors in a flow injection system with printed electrodes were developed and tested. A filter disc (7mm diameter) with immobilized enzyme was placed at the printed electrode. This conception combines the advantages of biosensors with a bioreceptor at the electrode surface and systems with spatially separated enzymatic and detection parts. Filters of different composition (glass, quartz, and cellulose), thickness, porosity, and ways of binding enzyme to their surface were tested. Only covalent bonds throughout a filter-aminosilane-glutaraldehyde-enzyme chain ensured a long-time and reproducible biosensor response. The developed method of biosensor preparation has been successfully applied to enzymes glucose oxidase, laccase and choline oxidase. The dependences of peak current on detection potential, flow rate, injection volume, analyte concentration as well as biosensor lifetime and reproducibility were investigated for glucose oxidase biosensor. The sensitivity of measurements was two or more times higher than that of biosensor with a mini-reactor filled by powder with immobilized enzyme. The developed biosensor with laccase was tested by determining dopamine in the pharmaceutical infusion product Tensamin. Results of the analysis (40.0±0.7mgmL-1, SD=0.8mgmL-1, RSD=1.85%, N=11) show a good agreement with the manufacturer's declared value.

Název v anglickém jazyce

Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes.

Popis výsledku anglicky

A new type of electrochemical biosensors in a flow injection system with printed electrodes were developed and tested. A filter disc (7mm diameter) with immobilized enzyme was placed at the printed electrode. This conception combines the advantages of biosensors with a bioreceptor at the electrode surface and systems with spatially separated enzymatic and detection parts. Filters of different composition (glass, quartz, and cellulose), thickness, porosity, and ways of binding enzyme to their surface were tested. Only covalent bonds throughout a filter-aminosilane-glutaraldehyde-enzyme chain ensured a long-time and reproducible biosensor response. The developed method of biosensor preparation has been successfully applied to enzymes glucose oxidase, laccase and choline oxidase. The dependences of peak current on detection potential, flow rate, injection volume, analyte concentration as well as biosensor lifetime and reproducibility were investigated for glucose oxidase biosensor. The sensitivity of measurements was two or more times higher than that of biosensor with a mini-reactor filled by powder with immobilized enzyme. The developed biosensor with laccase was tested by determining dopamine in the pharmaceutical infusion product Tensamin. Results of the analysis (40.0±0.7mgmL-1, SD=0.8mgmL-1, RSD=1.85%, N=11) show a good agreement with the manufacturer's declared value.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/GA23-07292S" target="_blank" >GA23-07292S: Aditivní nebo subtraktivní výroba: která z nich je účinnější pro dosažení stabilní elektroredukce oxidu uhličitého při vysokých reakčních rychlostech?</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

1878-562X

Svazek periodika

157

Číslo periodika v rámci svazku

JUN 2024

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

108663

Kód UT WoS článku

001183464500001

EID výsledku v databázi Scopus

2-s2.0-85185194393