Kinetika a specificita hydrolýzy oleje v superkritickém CO2 katalyzované Lipozymem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F08%3A00310266" target="_blank" >RIV/61388963:_____/08:00310266 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985858:_____/08:00310266

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Kinetics and Specificity of Lipozyme-Catalysed Oil Hydrolysis in Supercritical CO2

Popis výsledku v původním jazyce

Selective enzyme-catalysed hydrolysis of blackcurrant seed oil was studied with aim to obtain different levels of alpha- and/or gamma-linolenic acid in lipid classes in the hydrolyzate, making them suitable for special dietary needs. Supercritical CO2 with dissolved oil and water flowed through a packed bed reactor (temperature 40 °C, pressure 15-28 MPa, superficial velocity 0.1-0.7 mm/s) where Lipozyme, a 1,3-specific lipase from Mucor miehei was immobilised on a macroporous ion-exchange resin. The enzyme activity was stable as long as water precipitation in the reactor was prevented. The reaction was controlled by both Michaelis-Menten kinetics and mass transfer. The maximum rate of fatty acids liberation per unit amount of enzyme, 2.6 mmol/s kg, wasachieved at the maximum pressure and flow rate. The liberated fatty acids were enriched in alpha-linolenic, palmitic and stearic acids, while di- and monoacylglycerols contained increased levels of gamma-linolenic and stearidonic acids.

Název v anglickém jazyce

Kinetics and Specificity of Lipozyme-Catalysed Oil Hydrolysis in Supercritical CO2

Popis výsledku anglicky

Selective enzyme-catalysed hydrolysis of blackcurrant seed oil was studied with aim to obtain different levels of alpha- and/or gamma-linolenic acid in lipid classes in the hydrolyzate, making them suitable for special dietary needs. Supercritical CO2 with dissolved oil and water flowed through a packed bed reactor (temperature 40 °C, pressure 15-28 MPa, superficial velocity 0.1-0.7 mm/s) where Lipozyme, a 1,3-specific lipase from Mucor miehei was immobilised on a macroporous ion-exchange resin. The enzyme activity was stable as long as water precipitation in the reactor was prevented. The reaction was controlled by both Michaelis-Menten kinetics and mass transfer. The maximum rate of fatty acids liberation per unit amount of enzyme, 2.6 mmol/s kg, wasachieved at the maximum pressure and flow rate. The liberated fatty acids were enriched in alpha-linolenic, palmitic and stearic acids, while di- and monoacylglycerols contained increased levels of gamma-linolenic and stearidonic acids.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Engineering Research and Design

ISSN

0263-8762

e-ISSN

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Svazek periodika

86

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000258545800003

EID výsledku v databázi Scopus

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