Distinct modulation of telomere length in two T-lymphoblastic leukemia cell lines by cytotoxic nucleoside phosphonates PMEG and PMEDAP

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F10%3A00346304" target="_blank" >RIV/61388963:_____/10:00346304 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Distinct modulation of telomere length in two T-lymphoblastic leukemia cell lines by cytotoxic nucleoside phosphonates PMEG and PMEDAP

Popis výsledku v původním jazyce

We have previously shown that PMEG diphosphate and PMEDAP diphosphate inhibit the enzymatic activity of human telomerase in a cell-free assay. Here, we investigated the ability of PMEG and PMEDAP to induce telomere shortening and telomerase inhibition intwo T-cell leukemia cell lines. Both PMEDAP and PMEG induced telomere shortening in CCRF-CEM cells after 9 weeks of exposure while the effect of the compounds on telomere length in MOLT-4 cells was the opposite. No correlation between telomerase activity and telomere length was found. Both compounds also down-regulated the expression of hTERT and c-myc mRNA in both cell lines. In conclusion, PMEDAP and PMEG are able to modulate telomere length in leukemic cells and this effect is cell-type specific. Itis neither due to telomerase inhibition nor impairment of hTERT expression and it is likely to be telomerase-independent.

Název v anglickém jazyce

Distinct modulation of telomere length in two T-lymphoblastic leukemia cell lines by cytotoxic nucleoside phosphonates PMEG and PMEDAP

Popis výsledku anglicky

We have previously shown that PMEG diphosphate and PMEDAP diphosphate inhibit the enzymatic activity of human telomerase in a cell-free assay. Here, we investigated the ability of PMEG and PMEDAP to induce telomere shortening and telomerase inhibition intwo T-cell leukemia cell lines. Both PMEDAP and PMEG induced telomere shortening in CCRF-CEM cells after 9 weeks of exposure while the effect of the compounds on telomere length in MOLT-4 cells was the opposite. No correlation between telomerase activity and telomere length was found. Both compounds also down-regulated the expression of hTERT and c-myc mRNA in both cell lines. In conclusion, PMEDAP and PMEG are able to modulate telomere length in leukemic cells and this effect is cell-type specific. Itis neither due to telomerase inhibition nor impairment of hTERT expression and it is likely to be telomerase-independent.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Pharmacology

ISSN

0014-2999

e-ISSN

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Svazek periodika

643

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000280627700002

EID výsledku v databázi Scopus

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