Involvement of MAP Kinases in the Cytotoxicity of Acyclic Nucleoside Phosphonates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00376351" target="_blank" >RIV/61388963:_____/12:00376351 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Involvement of MAP Kinases in the Cytotoxicity of Acyclic Nucleoside Phosphonates

Popis výsledku v původním jazyce

The role of ERK, p38, JNK and AKT kinases in PMEG-induced apoptosis was investigated in CCRF-CEM and HL-60 leukemia cells. First, MAPK mRNA and protein expression were assessed in PMEG-treated cells. MAPK activation was then measured using phospho-specific antibodies. Up-regulation of p38beta, gamma and delta mRNA were observed following PMEG treatment of CCRF-CEM cells, however, the total protein expression remained unchanged. Neither PMEG nor its analogue 9-[2-(phosphonomethoxy)ethyl]-2,6-diaminopurine (PMEDAP) induced p38 kinase phosphorylation in CCRF-CEM cells, whereas increased p38 phosphorylation was observed in HL-60 cells. The ERK, JNK or AKT pathways did not. PMEG- and PMEDAP-induced cytotoxicity is therefore partly mediated by the p38 pathway in human leukemia cells.

Název v anglickém jazyce

Involvement of MAP Kinases in the Cytotoxicity of Acyclic Nucleoside Phosphonates

Popis výsledku anglicky

The role of ERK, p38, JNK and AKT kinases in PMEG-induced apoptosis was investigated in CCRF-CEM and HL-60 leukemia cells. First, MAPK mRNA and protein expression were assessed in PMEG-treated cells. MAPK activation was then measured using phospho-specific antibodies. Up-regulation of p38beta, gamma and delta mRNA were observed following PMEG treatment of CCRF-CEM cells, however, the total protein expression remained unchanged. Neither PMEG nor its analogue 9-[2-(phosphonomethoxy)ethyl]-2,6-diaminopurine (PMEDAP) induced p38 kinase phosphorylation in CCRF-CEM cells, whereas increased p38 phosphorylation was observed in HL-60 cells. The ERK, JNK or AKT pathways did not. PMEG- and PMEDAP-induced cytotoxicity is therefore partly mediated by the p38 pathway in human leukemia cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0508" target="_blank" >1M0508: Nová antivirotika a antineoplastika</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anticancer Research

ISSN

0250-7005

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

6

Strana od-do

497-502

Kód UT WoS článku

000299985800013

EID výsledku v databázi Scopus

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