Generalized polymer effective charge measurement by capillary isotachophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00435675" target="_blank" >RIV/61388963:_____/14:00435675 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2014.10.025" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2014.10.025</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2014.10.025" target="_blank" >10.1016/j.chroma.2014.10.025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Generalized polymer effective charge measurement by capillary isotachophoresis

Popis výsledku v původním jazyce

In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 mu m i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be similar to 5-10% for solutes with mobilities in the 20-50 x 10(-9) m(2) V-1 s(-1) range.

Název v anglickém jazyce

Generalized polymer effective charge measurement by capillary isotachophoresis

Popis výsledku anglicky

In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 mu m i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be similar to 5-10% for solutes with mobilities in the 20-50 x 10(-9) m(2) V-1 s(-1) range.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1370

Číslo periodika v rámci svazku

Nov 28

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

255-262

Kód UT WoS článku

000345724800029

EID výsledku v databázi Scopus

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