Cholesterol under oxidative stress ? How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00443291" target="_blank" >RIV/61388963:_____/15:00443291 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388955:_____/15:00443291

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.freeradbiomed.2015.03.006" target="_blank" >http://dx.doi.org/10.1016/j.freeradbiomed.2015.03.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.freeradbiomed.2015.03.006" target="_blank" >10.1016/j.freeradbiomed.2015.03.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cholesterol under oxidative stress ? How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Popis výsledku v původním jazyce

The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized ? tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.

Název v anglickém jazyce

Cholesterol under oxidative stress ? How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Popis výsledku anglicky

The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized ? tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP208%2F12%2FG016" target="_blank" >GBP208/12/G016: Řízení struktury a funkce biomolekul na molekulové úrovni: souhra teorie a experimentu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Free Radical Biology and Medicine

ISSN

0891-5849

e-ISSN

—

Svazek periodika

84

Číslo periodika v rámci svazku

JUL 2015

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

30-41

Kód UT WoS článku

000355896500004

EID výsledku v databázi Scopus

2-s2.0-84928530055