N-Branched acyclic nucleoside phosphonates as monomers for the synthesis of modified oligonucleotides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00444054" target="_blank" >RIV/61388963:_____/15:00444054 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c4ob02265c" target="_blank" >http://dx.doi.org/10.1039/c4ob02265c</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c4ob02265c" target="_blank" >10.1039/c4ob02265c</a>

Jazyk výsledku

angličtina

Název v původním jazyce

N-Branched acyclic nucleoside phosphonates as monomers for the synthesis of modified oligonucleotides

Popis výsledku v původním jazyce

Protected N-branched nucleoside phosphonates containing adenine and thymine bases were prepared as the monomers for the introduction of aza-acyclic nucleotide units into modified oligonucleotides. The phosphotriester and phosphoramidite methods were usedfor the incorporation of modified and natural units, respectively. The solid phase synthesis of a series of nonamers containing one central modified unit was successfully performed in both 3'-> 5' and 5'-> 3' directions. Hybridization properties of theprepared oligoribonucleotides and oligodeoxyribonucleotides were evaluated. The measurement of thermal characteristics of the complexes of modified nonamers with the complementary strand revealed a considerable destabilizing effect of the introduced units. We also examined the substrate/inhibitory properties of aza-acyclic nucleoside phosphono-diphosphate derivatives (analogues of nucleoside triphosphates) but neither inhibition of human and bacterial DNA polymerases nor polymerase-media

Název v anglickém jazyce

N-Branched acyclic nucleoside phosphonates as monomers for the synthesis of modified oligonucleotides

Popis výsledku anglicky

Protected N-branched nucleoside phosphonates containing adenine and thymine bases were prepared as the monomers for the introduction of aza-acyclic nucleotide units into modified oligonucleotides. The phosphotriester and phosphoramidite methods were usedfor the incorporation of modified and natural units, respectively. The solid phase synthesis of a series of nonamers containing one central modified unit was successfully performed in both 3'-> 5' and 5'-> 3' directions. Hybridization properties of theprepared oligoribonucleotides and oligodeoxyribonucleotides were evaluated. The measurement of thermal characteristics of the complexes of modified nonamers with the complementary strand revealed a considerable destabilizing effect of the introduced units. We also examined the substrate/inhibitory properties of aza-acyclic nucleoside phosphono-diphosphate derivatives (analogues of nucleoside triphosphates) but neither inhibition of human and bacterial DNA polymerases nor polymerase-media

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Organic & Biomolecular Chemistry

ISSN

1477-0520

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

4449-4458

Kód UT WoS článku

000352546100013

EID výsledku v databázi Scopus

2-s2.0-84926512826