Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00542326" target="_blank" >RIV/61388963:_____/21:00542326 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/ejoc.202100117" target="_blank" >https://doi.org/10.1002/ejoc.202100117</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ejoc.202100117" target="_blank" >10.1002/ejoc.202100117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels

Popis výsledku v původním jazyce

Cell analysis techniques like flow cytometry and fluorescence microscopy are widely used to explore cells in real‐time and provide important insights into a variety of cellular processes. These techniques require bright and specific staining reagents to generate strong fluorescence signal and enable reliable read‐out, making the choice of fluorescent labels a key aspect of experimental design. Much research has been done in the field of fluorophore development to generate bright small‐molecule dyes, fluorescent proteins, and emitting nanoparticles. However, it remains challenging to modify biomolecules with multiple emitters and avoid self‐quenching of such fluorophores at the same time. Herein, we present advances in multimerization‐based methods for the generation of fluorescent labels with high brightness and discuss their advantages and limitations in the context of flow cytometry and fluorescence microscopy applications.

Název v anglickém jazyce

Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels

Popis výsledku anglicky

Cell analysis techniques like flow cytometry and fluorescence microscopy are widely used to explore cells in real‐time and provide important insights into a variety of cellular processes. These techniques require bright and specific staining reagents to generate strong fluorescence signal and enable reliable read‐out, making the choice of fluorescent labels a key aspect of experimental design. Much research has been done in the field of fluorophore development to generate bright small‐molecule dyes, fluorescent proteins, and emitting nanoparticles. However, it remains challenging to modify biomolecules with multiple emitters and avoid self‐quenching of such fluorophores at the same time. Herein, we present advances in multimerization‐based methods for the generation of fluorescent labels with high brightness and discuss their advantages and limitations in the context of flow cytometry and fluorescence microscopy applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Organic Chemistry

ISSN

1434-193X

e-ISSN

1099-0690

Svazek periodika

2021

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

2817-2830

Kód UT WoS článku

000644543900001

EID výsledku v databázi Scopus

2-s2.0-85104988223