Radioactive Chernobyl Environment Has Produced High-Oil Flax Seeds That Show Proteome Alterations Related to Carbon Metabolism during Seed Development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00422907" target="_blank" >RIV/61388971:_____/13:00422907 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/pr400528m" target="_blank" >http://dx.doi.org/10.1021/pr400528m</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/pr400528m" target="_blank" >10.1021/pr400528m</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Radioactive Chernobyl Environment Has Produced High-Oil Flax Seeds That Show Proteome Alterations Related to Carbon Metabolism during Seed Development

Popis výsledku v původním jazyce

Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for this increase, seed development has been studied. Flax seeds were harvested in biological triplicate at 2, 4, and 6 weeks after flowering and at maturity from plants grown in nonradioactiveand radio-contaminated plots in the Chernobyl area for two generations. Quantitative proteomic analyses based on 2-D gel electrophoresis (2-DE) allowed us to establish developmental profiles for 199 2-DE spots in both plots, out of which 79 were reliably identified by tandem mass spectrometry. The data suggest a statistically significant increased abundance of proteins associated with pyruvate biosynthesis via cytoplasmic glycolysis, l-malate decarboxylation, isocitrate dehydrogenation,

Název v anglickém jazyce

Radioactive Chernobyl Environment Has Produced High-Oil Flax Seeds That Show Proteome Alterations Related to Carbon Metabolism during Seed Development

Popis výsledku anglicky

Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for this increase, seed development has been studied. Flax seeds were harvested in biological triplicate at 2, 4, and 6 weeks after flowering and at maturity from plants grown in nonradioactiveand radio-contaminated plots in the Chernobyl area for two generations. Quantitative proteomic analyses based on 2-D gel electrophoresis (2-DE) allowed us to establish developmental profiles for 199 2-DE spots in both plots, out of which 79 were reliably identified by tandem mass spectrometry. The data suggest a statistically significant increased abundance of proteins associated with pyruvate biosynthesis via cytoplasmic glycolysis, l-malate decarboxylation, isocitrate dehydrogenation,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Proteome Research

ISSN

1535-3893

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

4799-4806

Kód UT WoS článku

000326615000013

EID výsledku v databázi Scopus

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