Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00506696" target="_blank" >RIV/61388971:_____/14:00506696 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60193697:_____/14:#0000967

Výsledek na webu

<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.12610" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.12610</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jam.12610" target="_blank" >10.1111/jam.12610</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls

Popis výsledku v původním jazyce

AimsIn recent years, beer-spoilage cases from strictly anaerobic bacteria have risen in frequency, in connection with the production of non-pasteurized, non-alcohol and low-alcoholic beers and with the lowering of dissolved oxygen in the packaged beer. Selenomonas lacticifex, found in brewer's yeast and in biofilms covering some surfaces in brewery bottling area, is considered to be a beer-spoilage organism. This study aims to develop S.lacticifex-specific PCR assay. The objective of this study was also evaluation of the specificity and reproducibility of the developed PCR assay in real brewery samples. nMethods and ResultsThree primers (one forward and two reverse) were designed for identification of the strictly anaerobic bacterium S.lacticifex on the basis of the species-specific sequences of the 16S rDNA region. The specificity of the primers was tested against 44 brewery-related non-target micro-organisms that could potentially occur in the same brewery specimens. None of the primer pairs amplified DNA from any of the non-S.lacticifex strains tested including genera from the same family (Pectinatus, Megasphaera, Zymophilus) and the closely related species Selenomonas ruminantium, showing thus 100% specificity. nConclusionsThe PCR assay developed in this study enables the detection of the strictly anaerobic bacterium S.lacticifex in real brewery samples including pitching yeast. nSignificance and Impact of the StudySelenomonas lacticifex-specific PCR assay developed in this study allows for the extension of the spectra of detected beer-spoilage micro-organisms in brewing laboratories and thus lowering the risk of contamination of the final product.

Název v anglickém jazyce

Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls

Popis výsledku anglicky

AimsIn recent years, beer-spoilage cases from strictly anaerobic bacteria have risen in frequency, in connection with the production of non-pasteurized, non-alcohol and low-alcoholic beers and with the lowering of dissolved oxygen in the packaged beer. Selenomonas lacticifex, found in brewer's yeast and in biofilms covering some surfaces in brewery bottling area, is considered to be a beer-spoilage organism. This study aims to develop S.lacticifex-specific PCR assay. The objective of this study was also evaluation of the specificity and reproducibility of the developed PCR assay in real brewery samples. nMethods and ResultsThree primers (one forward and two reverse) were designed for identification of the strictly anaerobic bacterium S.lacticifex on the basis of the species-specific sequences of the 16S rDNA region. The specificity of the primers was tested against 44 brewery-related non-target micro-organisms that could potentially occur in the same brewery specimens. None of the primer pairs amplified DNA from any of the non-S.lacticifex strains tested including genera from the same family (Pectinatus, Megasphaera, Zymophilus) and the closely related species Selenomonas ruminantium, showing thus 100% specificity. nConclusionsThe PCR assay developed in this study enables the detection of the strictly anaerobic bacterium S.lacticifex in real brewery samples including pitching yeast. nSignificance and Impact of the StudySelenomonas lacticifex-specific PCR assay developed in this study allows for the extension of the spectra of detected beer-spoilage micro-organisms in brewing laboratories and thus lowering the risk of contamination of the final product.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

—

Svazek periodika

117

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1328-1335

Kód UT WoS článku

000343870700011

EID výsledku v databázi Scopus

2-s2.0-84908013133