Acrosin inhibitor detecting along the boar epididymis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00454044" target="_blank" >RIV/61388971:_____/16:00454044 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/16:00454044

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2015.10.034" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2015.10.034</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2015.10.034" target="_blank" >10.1016/j.ijbiomac.2015.10.034</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Acrosin inhibitor detecting along the boar epididymis

Popis výsledku v původním jazyce

Epididymal sperm maturation represents a key step in the reproduction process. Spermatozoa are exposed to epididymal fluid components representing the natural environment essential for their post-testicular maturation. Changes in sperm membrane proteinsare influenced by proteolytic, glycosylation and deglycosylation enzymes present in the epididymal fluid. Accordingly, the occurrence of inhibitors of these enzymes in the epididymis is very important for the regulation of sperm membrane protein processing. In the present study, we monitored acrosin inhibitor distribution in boar epididymal fluid and in spermatozoa from different segments of the organ. Using specific polyclonal antibody we registered increasing signal of the acrosin inhibitor (AI) fromcaput to cauda epididymis. Mass spectroscopy examination of the immunoprecipitated acrosin inhibitor (12kDa) unequivocally identified sperm-associated acrosin inhibitor (SAAI) in the epididymal tissue. Lectin staining showed N-glycosylati

Název v anglickém jazyce

Acrosin inhibitor detecting along the boar epididymis

Popis výsledku anglicky

Epididymal sperm maturation represents a key step in the reproduction process. Spermatozoa are exposed to epididymal fluid components representing the natural environment essential for their post-testicular maturation. Changes in sperm membrane proteinsare influenced by proteolytic, glycosylation and deglycosylation enzymes present in the epididymal fluid. Accordingly, the occurrence of inhibitors of these enzymes in the epididymis is very important for the regulation of sperm membrane protein processing. In the present study, we monitored acrosin inhibitor distribution in boar epididymal fluid and in spermatozoa from different segments of the organ. Using specific polyclonal antibody we registered increasing signal of the acrosin inhibitor (AI) fromcaput to cauda epididymis. Mass spectroscopy examination of the immunoprecipitated acrosin inhibitor (12kDa) unequivocally identified sperm-associated acrosin inhibitor (SAAI) in the epididymal tissue. Lectin staining showed N-glycosylati

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

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Svazek periodika

82

Číslo periodika v rámci svazku

Jan 2016

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

733-739

Kód UT WoS článku

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EID výsledku v databázi Scopus

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