Study of acrosin inhibitor along the boar epididymis

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of acrosin inhibitor along the boar epididymis

Popis výsledku v původním jazyce

Sperm maturation in the epididymis represents a key step in the reproduction process. Sperm plasma membrane is exposed to epididymal fluid representing the natural environment essential for their post-testicular maturation. Changes in the sperm membraneproteins are influenced by proteolytic and glycosidic enzymes present in the fluid. We monitored acrosin inhibitor (AI) in epididymal fluid and spermatozoa along the organ. Boar epididymal fluid with spermatozoa was isolated according to its spatial restriction into 9 fractions. The AI was detected in sperm extracts and fluids using specific polyclonal antibody. Expression of AI gene was investigated by PCR method. Additionally, immunofluorescence technique (IMF) was used for localization in the tissue.We registered the increasing signal of AI from caput to cauda epididymis. We confirmed that using gene expression method. Using specific antibody, AI was localized in the secretory tissues of caput, corpus and cauda epididymis by IMF.

Název v anglickém jazyce

Study of acrosin inhibitor along the boar epididymis

Popis výsledku anglicky

Sperm maturation in the epididymis represents a key step in the reproduction process. Sperm plasma membrane is exposed to epididymal fluid representing the natural environment essential for their post-testicular maturation. Changes in the sperm membraneproteins are influenced by proteolytic and glycosidic enzymes present in the fluid. We monitored acrosin inhibitor (AI) in epididymal fluid and spermatozoa along the organ. Boar epididymal fluid with spermatozoa was isolated according to its spatial restriction into 9 fractions. The AI was detected in sperm extracts and fluids using specific polyclonal antibody. Expression of AI gene was investigated by PCR method. Additionally, immunofluorescence technique (IMF) was used for localization in the tissue.We registered the increasing signal of AI from caput to cauda epididymis. We confirmed that using gene expression method. Using specific antibody, AI was localized in the secretory tissues of caput, corpus and cauda epididymis by IMF.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů