gamma-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542216" target="_blank" >RIV/61388971:_____/21:00542216 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2073-4409/10/4/776" target="_blank" >https://www.mdpi.com/2073-4409/10/4/776</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells10040776" target="_blank" >10.3390/cells10040776</a>

Jazyk výsledku

angličtina

Název v původním jazyce

gamma-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions

Popis výsledku v původním jazyce

Higher plants represent a large group of eukaryotes where centrosomes are absent. The functions of gamma-tubulin small complexes (gamma-TuSCs) and gamma-tubulin ring complexes (gamma-TuRCs) in metazoans and fungi in microtubule nucleation are well established and the majority of components found in the complexes are present in plants. However, plant microtubules are also nucleated in a gamma-tubulin-dependent but gamma-TuRC-independent manner. There is growing evidence that gamma-tubulin is a microtubule nucleator without being complexed in gamma-TuRC. Fibrillar arrays of gamma-tubulin were demonstrated in plant and animal cells and the ability of gamma-tubulin to assemble into linear oligomers/polymers was confirmed in vitro for both native and recombinant gamma-tubulin. The functions of gamma-tubulin as a template for microtubule nucleation or in promoting spontaneous nucleation is outlined. Higher plants represent an excellent model for studies on the role of gamma-tubulin in nucleation due to their acentrosomal nature and high abundancy and conservation of gamma-tubulin including its intrinsic ability to assemble filaments. The defining scaffolding or sequestration functions of plant gamma-tubulin in microtubule organization or in nuclear processes will help our understanding of its cellular roles in eukaryotes.

Název v anglickém jazyce

gamma-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions

Popis výsledku anglicky

Higher plants represent a large group of eukaryotes where centrosomes are absent. The functions of gamma-tubulin small complexes (gamma-TuSCs) and gamma-tubulin ring complexes (gamma-TuRCs) in metazoans and fungi in microtubule nucleation are well established and the majority of components found in the complexes are present in plants. However, plant microtubules are also nucleated in a gamma-tubulin-dependent but gamma-TuRC-independent manner. There is growing evidence that gamma-tubulin is a microtubule nucleator without being complexed in gamma-TuRC. Fibrillar arrays of gamma-tubulin were demonstrated in plant and animal cells and the ability of gamma-tubulin to assemble into linear oligomers/polymers was confirmed in vitro for both native and recombinant gamma-tubulin. The functions of gamma-tubulin as a template for microtubule nucleation or in promoting spontaneous nucleation is outlined. Higher plants represent an excellent model for studies on the role of gamma-tubulin in nucleation due to their acentrosomal nature and high abundancy and conservation of gamma-tubulin including its intrinsic ability to assemble filaments. The defining scaffolding or sequestration functions of plant gamma-tubulin in microtubule organization or in nuclear processes will help our understanding of its cellular roles in eukaryotes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cells

ISSN

2073-4409

e-ISSN

2073-4409

Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

776

Kód UT WoS článku

000642853900001

EID výsledku v databázi Scopus

2-s2.0-85105212260