Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00546810" target="_blank" >RIV/61388971:_____/21:00546810 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-021-92657-3" target="_blank" >https://www.nature.com/articles/s41598-021-92657-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-021-92657-3" target="_blank" >10.1038/s41598-021-92657-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment

Popis výsledku v původním jazyce

Algae are the main primary producers in aquatic environments and therefore of fundamental importance for the global ecosystem. Mid-infrared (IR) microspectroscopy is a non-invasive tool that allows in principle studying chemical composition on a single-cell level. For a long time, however, mid-infrared (IR) imaging of living algal cells in an aqueous environment has been a challenge due to the strong IR absorption of water. In this study, we employed multi-beam synchrotron radiation to measure time-resolved IR hyperspectral images of individual Thalassiosira weissflogii cells in water in the course of acclimation to an abrupt change of CO2 availability (from 390 to 5000 ppm and vice versa) over 75 min. We used a previously developed algorithm to correct sinusoidal interference fringes from IR hyperspectral imaging data. After preprocessing and fringe correction of the hyperspectral data, principal component analysis (PCA) was performed to assess the spatial distribution of organic pools within the algal cells. Through the analysis of 200,000 spectra, we were able to identify compositional modifications associated with CO2 treatment. PCA revealed changes in the carbohydrate pool (1200-950 cm(-1)), lipids (1740, 2852, 2922 cm(-1)), and nucleic acid (1160 and 1201 cm(-1)) as the major response of exposure to elevated CO2 concentrations. Our results show a local metabolism response to this external perturbation.

Název v anglickém jazyce

Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment

Popis výsledku anglicky

Algae are the main primary producers in aquatic environments and therefore of fundamental importance for the global ecosystem. Mid-infrared (IR) microspectroscopy is a non-invasive tool that allows in principle studying chemical composition on a single-cell level. For a long time, however, mid-infrared (IR) imaging of living algal cells in an aqueous environment has been a challenge due to the strong IR absorption of water. In this study, we employed multi-beam synchrotron radiation to measure time-resolved IR hyperspectral images of individual Thalassiosira weissflogii cells in water in the course of acclimation to an abrupt change of CO2 availability (from 390 to 5000 ppm and vice versa) over 75 min. We used a previously developed algorithm to correct sinusoidal interference fringes from IR hyperspectral imaging data. After preprocessing and fringe correction of the hyperspectral data, principal component analysis (PCA) was performed to assess the spatial distribution of organic pools within the algal cells. Through the analysis of 200,000 spectra, we were able to identify compositional modifications associated with CO2 treatment. PCA revealed changes in the carbohydrate pool (1200-950 cm(-1)), lipids (1740, 2852, 2922 cm(-1)), and nucleic acid (1160 and 1201 cm(-1)) as the major response of exposure to elevated CO2 concentrations. Our results show a local metabolism response to this external perturbation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-18917S" target="_blank" >GA17-18917S: Interakce řas a predátorů: Jak vycítit a reagovat na hrozbu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

11

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

13246

Kód UT WoS článku

000670729700023

EID výsledku v databázi Scopus

2-s2.0-85108678383