Critical Reviews and Perspectives beta-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00549270" target="_blank" >RIV/61388971:_____/21:00549270 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/nar/article/49/18/10221/6374178" target="_blank" >https://academic.oup.com/nar/article/49/18/10221/6374178</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkab803" target="_blank" >10.1093/nar/gkab803</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Critical Reviews and Perspectives beta-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck

Popis výsledku v původním jazyce

During the first step of gene expression, RNA polymerase (RNAP) engages DNA to transcribe RNA, forming highly stable complexes. These complexes need to be dissociated at the end of transcription units or when RNAP stalls during elongation and becomes an obstacle ('sitting duck') to further transcription or replication. In this review, we first outline the mechanisms involved in these processes. Then, we explore in detail the torpedo mechanism whereby a 5'-3' RNA exonuclease (torpedo) latches itself onto the 5' end of RNA protruding from RNAP, degrades it and upon contact with RNAP, induces dissociation of the complex. This mechanism, originally described in Eukaryotes and executed by Xrn-type 5'-3' exonucleases, was recently found in Bacteria and Archaea, mediated by beta-CASP family exonucleases. We discuss the mechanistic aspects of this process across the three kingdoms of life and conclude that 5'-3' exoribonucleases (beta-CASP and Xrn families) involved in the ancient torpedo mechanism have emerged at least twice during evolution.

Název v anglickém jazyce

Critical Reviews and Perspectives beta-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck

Popis výsledku anglicky

During the first step of gene expression, RNA polymerase (RNAP) engages DNA to transcribe RNA, forming highly stable complexes. These complexes need to be dissociated at the end of transcription units or when RNAP stalls during elongation and becomes an obstacle ('sitting duck') to further transcription or replication. In this review, we first outline the mechanisms involved in these processes. Then, we explore in detail the torpedo mechanism whereby a 5'-3' RNA exonuclease (torpedo) latches itself onto the 5' end of RNA protruding from RNAP, degrades it and upon contact with RNAP, induces dissociation of the complex. This mechanism, originally described in Eukaryotes and executed by Xrn-type 5'-3' exonucleases, was recently found in Bacteria and Archaea, mediated by beta-CASP family exonucleases. We discuss the mechanistic aspects of this process across the three kingdoms of life and conclude that 5'-3' exoribonucleases (beta-CASP and Xrn families) involved in the ancient torpedo mechanism have emerged at least twice during evolution.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA19-12956S" target="_blank" >GA19-12956S: Klíčové aspekty mykobakteriální transkriprce: SigA, podjednotka RNAP rozpoznávající promotor a její nově identifikovaný vazebný partner.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

1362-4962

Svazek periodika

49

Číslo periodika v rámci svazku

18

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

10221-10234

Kód UT WoS článku

000715870700008

EID výsledku v databázi Scopus

2-s2.0-85118286382