Sigma regulatory network in Rhodococcus erythropolis CCM2595

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00555955" target="_blank" >RIV/61388971:_____/22:00555955 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10442590

Výsledek na webu

<a href="https://academic.oup.com/femsle/article-abstract/369/1/fnac014/6528914?redirectedFrom=fulltext#no-access-message" target="_blank" >https://academic.oup.com/femsle/article-abstract/369/1/fnac014/6528914?redirectedFrom=fulltext#no-access-message</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsle/fnac014" target="_blank" >10.1093/femsle/fnac014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sigma regulatory network in Rhodococcus erythropolis CCM2595

Popis výsledku v původním jazyce

The aim of this investigation was to discover the promoters that drive expression of the sig genes encoding sigma factors of RNA polymerase in Rhodococcus erythropolis CCM2595 and classify these promoters according to the sigma factors which control their activity. To analyze the regulation of major sigma factors, which control large regulons that also contain genes expressed under exponential growth and non-stressed conditions, we used the R. erythropolis CCM2595 culture, which grew rapidly in minimal medium. The transcriptional start sites (TSSs) of the genes sigA, sigB, sigD, sigE, sigG, sigH, sigJ, and sigK were detected by primary 5 '-end-specific RNA sequencing. The promoters localized upstream of the detected TSSs were defined by their35 and10 elements, which were identical or closely similar to these sequences in the related species Corynebacterium glutamicum and Mycobacterium tuberculosis. Regulation of the promoter activities by different sigma factors was demonstrated by two independent techniques (in vivo and in vitro). All analyzed sig genes encoding the sigma factors with extracytoplasmic function (ECF) were found to be also driven from additional housekeeping promoters. Based on the classification of the sig gene promoters, a model of the basic sigma transcriptional regulatory network in R. erythropolis was designed.

Název v anglickém jazyce

Sigma regulatory network in Rhodococcus erythropolis CCM2595

Popis výsledku anglicky

The aim of this investigation was to discover the promoters that drive expression of the sig genes encoding sigma factors of RNA polymerase in Rhodococcus erythropolis CCM2595 and classify these promoters according to the sigma factors which control their activity. To analyze the regulation of major sigma factors, which control large regulons that also contain genes expressed under exponential growth and non-stressed conditions, we used the R. erythropolis CCM2595 culture, which grew rapidly in minimal medium. The transcriptional start sites (TSSs) of the genes sigA, sigB, sigD, sigE, sigG, sigH, sigJ, and sigK were detected by primary 5 '-end-specific RNA sequencing. The promoters localized upstream of the detected TSSs were defined by their35 and10 elements, which were identical or closely similar to these sequences in the related species Corynebacterium glutamicum and Mycobacterium tuberculosis. Regulation of the promoter activities by different sigma factors was demonstrated by two independent techniques (in vivo and in vitro). All analyzed sig genes encoding the sigma factors with extracytoplasmic function (ECF) were found to be also driven from additional housekeeping promoters. Based on the classification of the sig gene promoters, a model of the basic sigma transcriptional regulatory network in R. erythropolis was designed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA18-13254S" target="_blank" >GA18-13254S: Stresové odpovědi bakteriálního degradéra toxických polutantů Rhodococcus erythropolis</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

1574-6968

Svazek periodika

369

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

fnac014

Kód UT WoS článku

000764887700002

EID výsledku v databázi Scopus

2-s2.0-85125019957