Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00586459" target="_blank" >RIV/61388971:_____/24:00586459 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:90127/24:00139172 RIV/00216208:11310/24:10480909

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.analchem.3c04161" target="_blank" >10.1021/acs.analchem.3c04161</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting

Popis výsledku v původním jazyce

Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology.

Název v anglickém jazyce

Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting

Popis výsledku anglicky

Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LX22NPO5107" target="_blank" >LX22NPO5107: Národní ústav pro neurologický výzkum</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Chemistry

ISSN

0003-2700

e-ISSN

1520-6882

Svazek periodika

96

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

7386-7393

Kód UT WoS článku

001225426900001

EID výsledku v databázi Scopus

2-s2.0-85192254871