Accelerated dephosphorylation of adenosine phosphates and related compounds in the presence of nanocrystalline cerium oxide

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F16%3A00463206" target="_blank" >RIV/61388980:_____/16:00463206 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/44555601:13520/16:43887701 RIV/44555601:13440/16:43887701 RIV/61989592:15310/16:33161340

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c6en00086j" target="_blank" >http://dx.doi.org/10.1039/c6en00086j</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c6en00086j" target="_blank" >10.1039/c6en00086j</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Accelerated dephosphorylation of adenosine phosphates and related compounds in the presence of nanocrystalline cerium oxide

Popis výsledku v původním jazyce

Recently, cerium oxide has been used in many exciting applications in biology and medicine, where its enzyme-mimetic abilities can be exploited. A more in-depth understanding of its interactions with biologically relevant molecules is highly desirable. It is shown in this study that certain forms of cerium oxide, namely, the nanocrystalline cerium oxide (nanoceria) prepared by the thermal decomposition of cerium carbonate, may exhibit a remarkable ability to promote the dephosphorylation reactions of nucleotides and related compounds -adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), cytidine monophosphate (CMP), guanosine monophosphate (GMP), thiamine pyrophosphate (TPP) and thiamine monophosphate (TMP). The dephosphorylation proceeded under relatively mild (ambient) conditions with a rate constant proportional to the concentration of nanoceria and was almost independent of pH. The phosphoester bonds were cleaved through nucleophilic substitution, utilizing the surface hydroxyl groups as nucleophilic agents, whereas the electrophilicity of the phosphorus atoms was enhanced by the Lewis acid effect of the cerium cations. Moreover, more complex biologically relevant molecules may be subjected to degradation in the presence of cerium oxide, namely, a-nicotinamide adenine dinucleotide (NAD). The key characteristics of cerium oxide (surface area, crystallinity) as well as its surface chemistry and dephosphorylation activity are governed by the calcination temperature -the most effective cerium oxides were prepared by annealing at temperatures below ca. 600 degrees C. Notably, certain commercially available cerium oxides exhibited even higher dephosphorylation activity than the in-house nanoceria.

Název v anglickém jazyce

Accelerated dephosphorylation of adenosine phosphates and related compounds in the presence of nanocrystalline cerium oxide

Popis výsledku anglicky

Recently, cerium oxide has been used in many exciting applications in biology and medicine, where its enzyme-mimetic abilities can be exploited. A more in-depth understanding of its interactions with biologically relevant molecules is highly desirable. It is shown in this study that certain forms of cerium oxide, namely, the nanocrystalline cerium oxide (nanoceria) prepared by the thermal decomposition of cerium carbonate, may exhibit a remarkable ability to promote the dephosphorylation reactions of nucleotides and related compounds -adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), cytidine monophosphate (CMP), guanosine monophosphate (GMP), thiamine pyrophosphate (TPP) and thiamine monophosphate (TMP). The dephosphorylation proceeded under relatively mild (ambient) conditions with a rate constant proportional to the concentration of nanoceria and was almost independent of pH. The phosphoester bonds were cleaved through nucleophilic substitution, utilizing the surface hydroxyl groups as nucleophilic agents, whereas the electrophilicity of the phosphorus atoms was enhanced by the Lewis acid effect of the cerium cations. Moreover, more complex biologically relevant molecules may be subjected to degradation in the presence of cerium oxide, namely, a-nicotinamide adenine dinucleotide (NAD). The key characteristics of cerium oxide (surface area, crystallinity) as well as its surface chemistry and dephosphorylation activity are governed by the calcination temperature -the most effective cerium oxides were prepared by annealing at temperatures below ca. 600 degrees C. Notably, certain commercially available cerium oxides exhibited even higher dephosphorylation activity than the in-house nanoceria.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CA - Anorganická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/LM2015073" target="_blank" >LM2015073: Nanomateriály a nanotechnologie pro ochranu životního prostředí a udržitelnou budoucnost</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Science-Nano

ISSN

2051-8153

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

847-856

Kód UT WoS článku

000381438900018

EID výsledku v databázi Scopus

2-s2.0-84981294020