Evaluation of Brain Nuclear Medicine Imaging Tracers in a Murine Model of Sepsis-Associated Encephalopathy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F18%3A00497249" target="_blank" >RIV/61389005:_____/18:00497249 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11307-018-1201-3" target="_blank" >http://dx.doi.org/10.1007/s11307-018-1201-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11307-018-1201-3" target="_blank" >10.1007/s11307-018-1201-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of Brain Nuclear Medicine Imaging Tracers in a Murine Model of Sepsis-Associated Encephalopathy

Popis výsledku v původním jazyce

The purpose of this study was to evaluate a set of widely used nuclear medicine imaging agents as possible methods to study the early effects of systemic inflammation on the living brain in a mouse model of sepsis-associated encephalopathy (SAE). The lipopolysaccharide (LPS)-induced murine systemic inflammation model was selected as a model of SAE.ProceduresC57BL/6 mice were used. A multimodal imaging protocol was carried out on each animal 4h following the intravenous administration of LPS using the following tracers: [Tc-99m][2,2-dimethyl-3-[(3E)-3-oxidoiminobutan-2-yl]azanidylpropyl]-[(3E)-3-hydroxyiminobutan-2-yl]azanide ([Tc-99m]HMPAO) and ethyl-7-[I-125]iodo-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate ([I-125]iomazenil) to measure brain perfusion and neuronal damage, respectively, 2-deoxy-2-[F-18]fluoro-d-glucose ([F-18]FDG) to measure cerebral glucose uptake. We assessed microglia activity on another group of mice using 2-[6-chloro-2-(4-[I-125]iodophenyl)-imidazo[1,2-a]pyridin-3-yl]-N-ethyl-N-methyl-acetamide ([I-125]CLINME). Radiotracer uptakes were measured in different brain regions and correlated. Microglia activity was also assessed using immunohistochemistry. Brain glutathione levels were measured to investigate oxidative stress.

Název v anglickém jazyce

Evaluation of Brain Nuclear Medicine Imaging Tracers in a Murine Model of Sepsis-Associated Encephalopathy

Popis výsledku anglicky

The purpose of this study was to evaluate a set of widely used nuclear medicine imaging agents as possible methods to study the early effects of systemic inflammation on the living brain in a mouse model of sepsis-associated encephalopathy (SAE). The lipopolysaccharide (LPS)-induced murine systemic inflammation model was selected as a model of SAE.ProceduresC57BL/6 mice were used. A multimodal imaging protocol was carried out on each animal 4h following the intravenous administration of LPS using the following tracers: [Tc-99m][2,2-dimethyl-3-[(3E)-3-oxidoiminobutan-2-yl]azanidylpropyl]-[(3E)-3-hydroxyiminobutan-2-yl]azanide ([Tc-99m]HMPAO) and ethyl-7-[I-125]iodo-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate ([I-125]iomazenil) to measure brain perfusion and neuronal damage, respectively, 2-deoxy-2-[F-18]fluoro-d-glucose ([F-18]FDG) to measure cerebral glucose uptake. We assessed microglia activity on another group of mice using 2-[6-chloro-2-(4-[I-125]iodophenyl)-imidazo[1,2-a]pyridin-3-yl]-N-ethyl-N-methyl-acetamide ([I-125]CLINME). Radiotracer uptakes were measured in different brain regions and correlated. Microglia activity was also assessed using immunohistochemistry. Brain glutathione levels were measured to investigate oxidative stress.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Imaging and Biology

ISSN

1536-1632

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

952-962

Kód UT WoS článku

000450173100007

EID výsledku v databázi Scopus

2-s2.0-85046534861