Variations among glioblastoma cell lines in boron-mediated enhancement of cell killing by proton beams

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F25%3A00638313" target="_blank" >RIV/61389005:_____/25:00638313 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00177016:_____/25:N0000089 RIV/00064211:_____/25:W0000073

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-025-14658-w" target="_blank" >https://www.nature.com/articles/s41598-025-14658-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-14658-w" target="_blank" >10.1038/s41598-025-14658-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Variations among glioblastoma cell lines in boron-mediated enhancement of cell killing by proton beams

Popis výsledku v původním jazyce

Enhancement in cell killing by proton beams in the presence of boron (natural mixture natB: 80% 11B, 20% 10B) was reported, selectively in the Bragg peak region, putatively due to the proton-11B capture reaction. However, as some groups observed no such enhancement or assigned it to secondary neutron-10B capture, proton-boron capture therapy (PBCT) remains controversial. We previously validated this concept for U-87 MG glioblastoma cells. To test its generality and potential applicability for these tumours, we assessed PBCT using three further cell lines widely used in glioblastoma research. In U251 cells, natB enhanced cell killing by protons in Bragg peak but also in plateau regions, effects of 10B were even higher, and were found also for 18MV but not 6 MV photon beams (above and below photo-neutron production thresholds, respectively), suggesting a key role of secondary neutrons. For A172 and T98G cells, no enhancement was found at all. This variability among cell lines may stem from differences in boron uptake and/or in intercellular signalling likely needed to amplify the initial events in a few hit cells to population-level effects. Together with recent negative studies, the results suggest that potential clinical applications of PBCT are less promising than originally thought.

Název v anglickém jazyce

Variations among glioblastoma cell lines in boron-mediated enhancement of cell killing by proton beams

Popis výsledku anglicky

Enhancement in cell killing by proton beams in the presence of boron (natural mixture natB: 80% 11B, 20% 10B) was reported, selectively in the Bragg peak region, putatively due to the proton-11B capture reaction. However, as some groups observed no such enhancement or assigned it to secondary neutron-10B capture, proton-boron capture therapy (PBCT) remains controversial. We previously validated this concept for U-87 MG glioblastoma cells. To test its generality and potential applicability for these tumours, we assessed PBCT using three further cell lines widely used in glioblastoma research. In U251 cells, natB enhanced cell killing by protons in Bragg peak but also in plateau regions, effects of 10B were even higher, and were found also for 18MV but not 6 MV photon beams (above and below photo-neutron production thresholds, respectively), suggesting a key role of secondary neutrons. For A172 and T98G cells, no enhancement was found at all. This variability among cell lines may stem from differences in boron uptake and/or in intercellular signalling likely needed to amplify the initial events in a few hit cells to population-level effects. Together with recent negative studies, the results suggest that potential clinical applications of PBCT are less promising than originally thought.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA21-06451S" target="_blank" >GA21-06451S: Záhada zvýšení buněčné odezvy indukované protony v přítomnosti boru</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

29453

Kód UT WoS článku

001548644500004

EID výsledku v databázi Scopus

2-s2.0-105013258275