Excretome of the chitinolytic bacterium Clostridium paraputrificum J4

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00387248" target="_blank" >RIV/61389013:_____/12:00387248 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/12:00387248

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12223-012-0137-2" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0137-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-012-0137-2" target="_blank" >10.1007/s12223-012-0137-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Excretome of the chitinolytic bacterium Clostridium paraputrificum J4

Popis výsledku v původním jazyce

A strictly anaerobic mesophilic chitinolytic bacterial strain identified as Clostridium paraputrificum J4 was isolated from human feces. In response to various types of growth substrates, the bacterium produced an array of chitinolytic enzymes representing significant components of the J4 strain secretome. The excreted active proteins were characterized by estimating the enzymatic activities of endochitinase, exochitinase, and N-acetylglucosaminidase induced by cultivation in medium M-10 with colloidalchitin. The enzyme activities produced by J4 strain cultivated in medium M-10 with glucose were significantly lower. The spectrum of extracellularly excreted proteins was separated by SDS-PAGE. The chitinase variability was confirmed on zymograms of renatured SDS-PAGE. The enzymes were visualized under ultraviolet light by using 4-methylumbelliferyl derivatives of N-acetyl-beta-d-glucosaminide, N,NA '-diacetyl-beta-d-chitobiose, or N,NA ',NEe-triacetyl-beta-d-chitotriose for beta-N-acety

Název v anglickém jazyce

Excretome of the chitinolytic bacterium Clostridium paraputrificum J4

Popis výsledku anglicky

A strictly anaerobic mesophilic chitinolytic bacterial strain identified as Clostridium paraputrificum J4 was isolated from human feces. In response to various types of growth substrates, the bacterium produced an array of chitinolytic enzymes representing significant components of the J4 strain secretome. The excreted active proteins were characterized by estimating the enzymatic activities of endochitinase, exochitinase, and N-acetylglucosaminidase induced by cultivation in medium M-10 with colloidalchitin. The enzyme activities produced by J4 strain cultivated in medium M-10 with glucose were significantly lower. The spectrum of extracellularly excreted proteins was separated by SDS-PAGE. The chitinase variability was confirmed on zymograms of renatured SDS-PAGE. The enzymes were visualized under ultraviolet light by using 4-methylumbelliferyl derivatives of N-acetyl-beta-d-glucosaminide, N,NA '-diacetyl-beta-d-chitobiose, or N,NA ',NEe-triacetyl-beta-d-chitotriose for beta-N-acety

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F09%2F1407" target="_blank" >GA310/09/1407: Chitinolytické enzymy z bakterie Clostridium paraputrificum sídlící v trávícím traktu člověka; struktura a biolékařské důsledky.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

335-339

Kód UT WoS článku

000305980400019

EID výsledku v databázi Scopus

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