Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F02%3A21023078" target="_blank" >RIV/67985904:_____/02:21023078 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract.

Popis výsledku v původním jazyce

A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum.The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas and formation of acetate and lactate and only small quantities of propionate and butyrate. In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d. The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90 of chitin added was degraded within 2 d of cultivation. On the zymogram of the extracelular chitinolytic complex were visible at least 6 isoenzymes with molar m

Název v anglickém jazyce

Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract.

Popis výsledku anglicky

A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum.The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas and formation of acetate and lactate and only small quantities of propionate and butyrate. In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d. The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90 of chitin added was degraded within 2 d of cultivation. On the zymogram of the extracelular chitinolytic complex were visible at least 6 isoenzymes with molar m

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

559-564

Kód UT WoS článku

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EID výsledku v databázi Scopus

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