Chitinolytic bacterial populations in gastrointestinal tract of wild ruminants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F12%3A00202761" target="_blank" >RIV/68407700:21460/12:00202761 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Chitinolytic bacterial populations in gastrointestinal tract of wild ruminants

Popis výsledku v původním jazyce

In this study we focused on bacteria from the animal digestive tract with an ability to utilize chitin. As cultivation media we used M-10 medium with colloid chitin as source of energy. Cultivation was carried out under anaerobic conditions. Chitinolyticbacteria were isolated from enrichment culture with faeces of different wild ruminants (fallow deer, Pere David's deer, Roth schild's giraffe). Isolates were selected accoring to their activity towards CM chitin and then characterized. The identification of strains with chitinolytic activities was made due to sequencing method. For further characterization of biochemical ability to degrade chitin substrate we will perform polyacrylamide gel electrophoresis of proteins, which are produced by chitosan degradation. The visualization of the chitinolytic enzymes will be achieved by zymography technique.

Název v anglickém jazyce

Chitinolytic bacterial populations in gastrointestinal tract of wild ruminants

Popis výsledku anglicky

In this study we focused on bacteria from the animal digestive tract with an ability to utilize chitin. As cultivation media we used M-10 medium with colloid chitin as source of energy. Cultivation was carried out under anaerobic conditions. Chitinolyticbacteria were isolated from enrichment culture with faeces of different wild ruminants (fallow deer, Pere David's deer, Roth schild's giraffe). Isolates were selected accoring to their activity towards CM chitin and then characterized. The identification of strains with chitinolytic activities was made due to sequencing method. For further characterization of biochemical ability to degrade chitin substrate we will perform polyacrylamide gel electrophoresis of proteins, which are produced by chitosan degradation. The visualization of the chitinolytic enzymes will be achieved by zymography technique.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Instuments and Methods for Biology and Medicine 2012

ISBN

978-80-01-05119-1

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

42-45

Název nakladatele

Czech Technical University

Místo vydání

Prague

Místo konání akce

Kladno

Datum konání akce

29. 5. 2012

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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