Leucine modifications by He/O2 plasma treatment in phosphate-buffered saline: Bactericidal effects and chemical characterization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F21%3A00555846" target="_blank" >RIV/61389021:_____/21:00555846 - isvavai.cz</a>

Výsledek na webu

<a href="https://iopscience.iop.org/article/10.1088/1361-6463/ac252e" target="_blank" >https://iopscience.iop.org/article/10.1088/1361-6463/ac252e</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6463/ac252e" target="_blank" >10.1088/1361-6463/ac252e</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Leucine modifications by He/O2 plasma treatment in phosphate-buffered saline: Bactericidal effects and chemical characterization

Popis výsledku v původním jazyce

We studied the chemical and bactericidal properties of plasma-modified amino acid leucine (Leu) treated in phosphate-buffered saline (PBS) using an atmospheric-pressure plasma jet operating with He/O2 gas. We determined the initial formation of hypochlorite by the reaction of plasma-supplied O atoms with chloride ions in PBS, followed by the subsequent chlorination of Leu. The principal chemical process involved the formation of Leu monochloramine and Leu dichloramine. The products of plasma-modified Leu further possessed post-discharge reactivity characterized by the decay of Leu chloramines to the secondary products, i.e. isovaleraldehyde, isovaleronitrile, and N-chloromethyl-3-butaldimine. Our study of the effects of Leu on the viability of Escherichia coli bacteria in the plasma-treated PBS solution demonstrated time-and pH-dependent cytotoxic behavior, which were associated with the formation of different reaction products. At shorter treatment times, the addition of Leu hindered the bactericidal properties of plasma-treated PBS caused by the consumption of bactericidal hypochlorite in reaction with Leu and formation of non-bactericidal Leu monochloramine. Acidification to pH = 3 and longer plasma treatment substantially enhanced the bactericidal effects of Leu solutions, which were tentatively assigned to the formation of Leu dichloramine.

Název v anglickém jazyce

Leucine modifications by He/O2 plasma treatment in phosphate-buffered saline: Bactericidal effects and chemical characterization

Popis výsledku anglicky

We studied the chemical and bactericidal properties of plasma-modified amino acid leucine (Leu) treated in phosphate-buffered saline (PBS) using an atmospheric-pressure plasma jet operating with He/O2 gas. We determined the initial formation of hypochlorite by the reaction of plasma-supplied O atoms with chloride ions in PBS, followed by the subsequent chlorination of Leu. The principal chemical process involved the formation of Leu monochloramine and Leu dichloramine. The products of plasma-modified Leu further possessed post-discharge reactivity characterized by the decay of Leu chloramines to the secondary products, i.e. isovaleraldehyde, isovaleronitrile, and N-chloromethyl-3-butaldimine. Our study of the effects of Leu on the viability of Escherichia coli bacteria in the plasma-treated PBS solution demonstrated time-and pH-dependent cytotoxic behavior, which were associated with the formation of different reaction products. At shorter treatment times, the addition of Leu hindered the bactericidal properties of plasma-treated PBS caused by the consumption of bactericidal hypochlorite in reaction with Leu and formation of non-bactericidal Leu monochloramine. Acidification to pH = 3 and longer plasma treatment substantially enhanced the bactericidal effects of Leu solutions, which were tentatively assigned to the formation of Leu dichloramine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10305 - Fluids and plasma physics (including surface physics)

Projekt

<a href="/cs/project/GA19-25026S" target="_blank" >GA19-25026S: Charakterizace chemicky reaktivních kapalin produkovaných nerovnovážným plazmatem</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physics D-Applied Physics

ISSN

0022-3727

e-ISSN

1361-6463

Svazek periodika

54

Číslo periodika v rámci svazku

50

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

505206

Kód UT WoS článku

000701256000001

EID výsledku v databázi Scopus

2-s2.0-85116879960