Specificity of MOAC enrichment applied for mature pollen phosphoproteomics studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00387762" target="_blank" >RIV/61389030:_____/12:00387762 - isvavai.cz</a>

Výsledek na webu

<a href="http://home.ueb.cas.cz/publikace/2012_Fila_IEEE.pdf" target="_blank" >http://home.ueb.cas.cz/publikace/2012_Fila_IEEE.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/iCBEB.2012.362" target="_blank" >10.1109/iCBEB.2012.362</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Specificity of MOAC enrichment applied for mature pollen phosphoproteomics studies

Popis výsledku v původním jazyce

Androgenesis represents one of the most important and fast-developing biotechnological approaches for the production of haploid and di-haploid crop plants with great potential for plant breeding. Tobacco serves as a suitable model plant with available protocols for androgenesis. However, it is necessary to understand the molecular regulatory mechanisms underlying this process. Phosphorylation is the most dynamic posttranslational modification. In a time, the cell contains only few percent of phosphoproteins that can furthermore be accompanied by their native forms. Thus, the enrichment techniques have to be applied in order to concentrate the phosphorylated forms. The aim of our studies was to compare the phosphoproteins extracted by TCA/acetone out ofmature pollen and 30-min-activated pollen. The crude protein extracts were enriched by the metaloxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix. The efficiency of the enrichment was verified by 1D SDS-PAGE

Název v anglickém jazyce

Specificity of MOAC enrichment applied for mature pollen phosphoproteomics studies

Popis výsledku anglicky

Androgenesis represents one of the most important and fast-developing biotechnological approaches for the production of haploid and di-haploid crop plants with great potential for plant breeding. Tobacco serves as a suitable model plant with available protocols for androgenesis. However, it is necessary to understand the molecular regulatory mechanisms underlying this process. Phosphorylation is the most dynamic posttranslational modification. In a time, the cell contains only few percent of phosphoproteins that can furthermore be accompanied by their native forms. Thus, the enrichment techniques have to be applied in order to concentrate the phosphorylated forms. The aim of our studies was to compare the phosphoproteins extracted by TCA/acetone out ofmature pollen and 30-min-activated pollen. The crude protein extracts were enriched by the metaloxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix. The efficiency of the enrichment was verified by 1D SDS-PAGE

Druh

D - Stať ve sborníku

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceeding of International Conference on Biomedical Engineering and Biotechnology

ISBN

978-0-7695-4706-0

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

523-526

Název nakladatele

IEEE Computer Society

Místo vydání

Washington, D.C

Místo konání akce

Macau

Datum konání akce

28. 5. 2012

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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