In vitro antiinflammatory and antioxidant potential of root extracts from Ranunculaceae species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476506" target="_blank" >RIV/61389030:_____/17:00476506 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/17:71600 RIV/60460709:41340/17:71600

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.sajb.2016.12.008" target="_blank" >http://dx.doi.org/10.1016/j.sajb.2016.12.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sajb.2016.12.008" target="_blank" >10.1016/j.sajb.2016.12.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro antiinflammatory and antioxidant potential of root extracts from Ranunculaceae species

Popis výsledku v původním jazyce

Among the ethanol extracts of roots of 25 Ranunculaceous plant species traditionally used in treatment of various inflammatory diseases, Helleborus purpurascens demonstrated the most promising inhibitory activity towards COX-1, COX-2 and 5-LOX enzymes (IC 50 detected as 5.64, 10.33 and 3.84 μg/mL, respectively). GC–MS analysis of subfractions obtained from H. purpurascens extract revealed that a complex of fatty acids (especially α-linolenic acid) might be responsible for the observed antiinflammatory activity. The extract of Thalictrum minus was found to be selectively inhibiting COX-2 and 5-LOX enzymes (IC 50  = 49.68 and 76.47 μg/mL, respectively) but had no inhibitory effect on COX-1 (IC 50   >  128 μg/mL). Moreover, T. minus extract also showed significant antioxidant effect (DPPH = 0.074 g TE/g, and ORAC = 0.518 g TE/g) as well as was relatively rich on phenolic compounds (TPC = 0.352 g GAE/g). Statistical analysis showed that phenolic compounds might be only partially responsible for antioxidant potential demonstrated by this plant. The results of our study suggest that the above-mentioned members of Ranunculaceae family may possibly serve as a prospective material for further development of novel and safer plant-based antiinflammatory and/or antioxidant agents.

Název v anglickém jazyce

In vitro antiinflammatory and antioxidant potential of root extracts from Ranunculaceae species

Popis výsledku anglicky

Among the ethanol extracts of roots of 25 Ranunculaceous plant species traditionally used in treatment of various inflammatory diseases, Helleborus purpurascens demonstrated the most promising inhibitory activity towards COX-1, COX-2 and 5-LOX enzymes (IC 50 detected as 5.64, 10.33 and 3.84 μg/mL, respectively). GC–MS analysis of subfractions obtained from H. purpurascens extract revealed that a complex of fatty acids (especially α-linolenic acid) might be responsible for the observed antiinflammatory activity. The extract of Thalictrum minus was found to be selectively inhibiting COX-2 and 5-LOX enzymes (IC 50  = 49.68 and 76.47 μg/mL, respectively) but had no inhibitory effect on COX-1 (IC 50   >  128 μg/mL). Moreover, T. minus extract also showed significant antioxidant effect (DPPH = 0.074 g TE/g, and ORAC = 0.518 g TE/g) as well as was relatively rich on phenolic compounds (TPC = 0.352 g GAE/g). Statistical analysis showed that phenolic compounds might be only partially responsible for antioxidant potential demonstrated by this plant. The results of our study suggest that the above-mentioned members of Ranunculaceae family may possibly serve as a prospective material for further development of novel and safer plant-based antiinflammatory and/or antioxidant agents.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA16-07193S" target="_blank" >GA16-07193S: Protizánětlivá aktivita vybraných stilbenů, 2-arylbenzofuranů a jejich metabolitů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

South African Journal of Botany

ISSN

0254-6299

e-ISSN

—

Svazek periodika

109

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

ZA - Jihoafrická republika

Počet stran výsledku

10

Strana od-do

128-137

Kód UT WoS článku

000400465400016

EID výsledku v databázi Scopus

2-s2.0-85009121766