Transcriptomic Response of Arabidopsis thaliana Exposed to CuO Nanoparticles, Bulk Material, and Ionic Copper

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00479928" target="_blank" >RIV/61389030:_____/17:00479928 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985858:_____/17:00479928 RIV/00216208:11310/17:10370366

Výsledek na webu

<a href="http://dx.doi.org/10.1021/acs.est.7b02265" target="_blank" >http://dx.doi.org/10.1021/acs.est.7b02265</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.est.7b02265" target="_blank" >10.1021/acs.est.7b02265</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transcriptomic Response of Arabidopsis thaliana Exposed to CuO Nanoparticles, Bulk Material, and Ionic Copper

Popis výsledku v původním jazyce

Engineered nanoparticles (ENPs) exhibit unique properties advantageous in a number of applications, but they also represent potential health and environmental risks. In this study, we investigated the phytotoxic mechanism of CuO ENPs using transcriptomic analysis and compared this response with the response to CuO bulk particles and ionic Cu 2+ . Ionic Cu 2+ at the concentration of 0.16 mg L1 changed transcription of 2692 genes (p value of < 0.001, fold change of ≥2) after 7 days of exposure, whereas CuO ENPs and bulk particles (both in the concentration of 10 mg L1 ) altered the expression of 922 and 482 genes in Arabidopsis thaliana roots, respectively. The similarity between transcription profiles of plants exposed to ENPs and ionic Cu 2+ indicated that the main factor in phytotoxicity was the release of Cu 2+ ions from CuO ENPs after 7 days of exposure. The effect of Cu 2+ ions was evident in all treatments, as indicated by the down-regulation of genes involved in metal homeostasis and transport and the up-regulation of oxidative stress response genes. ENPs were more soluble than bulk particles, resulting in the up-regulation of metallochaperone-like genes or the down-regulation of aquaporins and metal transmembrane transporters that was also characteristic for ionic Cu 2+ exposure.

Název v anglickém jazyce

Transcriptomic Response of Arabidopsis thaliana Exposed to CuO Nanoparticles, Bulk Material, and Ionic Copper

Popis výsledku anglicky

Engineered nanoparticles (ENPs) exhibit unique properties advantageous in a number of applications, but they also represent potential health and environmental risks. In this study, we investigated the phytotoxic mechanism of CuO ENPs using transcriptomic analysis and compared this response with the response to CuO bulk particles and ionic Cu 2+ . Ionic Cu 2+ at the concentration of 0.16 mg L1 changed transcription of 2692 genes (p value of < 0.001, fold change of ≥2) after 7 days of exposure, whereas CuO ENPs and bulk particles (both in the concentration of 10 mg L1 ) altered the expression of 922 and 482 genes in Arabidopsis thaliana roots, respectively. The similarity between transcription profiles of plants exposed to ENPs and ionic Cu 2+ indicated that the main factor in phytotoxicity was the release of Cu 2+ ions from CuO ENPs after 7 days of exposure. The effect of Cu 2+ ions was evident in all treatments, as indicated by the down-regulation of genes involved in metal homeostasis and transport and the up-regulation of oxidative stress response genes. ENPs were more soluble than bulk particles, resulting in the up-regulation of metallochaperone-like genes or the down-regulation of aquaporins and metal transmembrane transporters that was also characteristic for ionic Cu 2+ exposure.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Science and Technology

ISSN

0013-936X

e-ISSN

—

Svazek periodika

51

Číslo periodika v rámci svazku

18

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

10814-10824

Kód UT WoS článku

000411549800060

EID výsledku v databázi Scopus

2-s2.0-85029690803