EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00582673" target="_blank" >RIV/61389030:_____/24:00582673 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26220/23:PU149505 RIV/61989592:15310/24:73625406

Výsledek na webu

<a href="https://doi.org/10.1007/s00425-023-04293-y" target="_blank" >https://doi.org/10.1007/s00425-023-04293-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00425-023-04293-y" target="_blank" >10.1007/s00425-023-04293-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder

Popis výsledku v původním jazyce

Preparation and mounting of the samples are key factors for successful live cell microscopy. To acquire biologically relevant data, it is necessary to minimize stress and avoid physical damage to plant tissues during the installation of the sample into the microscope. This is challenging, particularly when the whole plant is mounted as the living sample needs to be properly anchored in the microscopic system to obtain high-quality and high-resolution data. Here, we present a user-optimized sample holder EasyClick for live cell inverted confocal microscopic analysis of plant roots with diameters from 0.3 to 0.7 mm. The EasyClick holder was tested on an inverted confocal microscope using germinating plants of several cereals. Nevertheless, it can be directly used on other types of inverted microscopes from various producers and on different plant species. The EasyClick holder effectively restricts root lateral and vertical movements. This greatly improves the conditions for time-lapse microscopy of the samples of interest.

Název v anglickém jazyce

EasyClick: an improved system for confocal microscopy of live roots with a user-optimized sample holder

Popis výsledku anglicky

Preparation and mounting of the samples are key factors for successful live cell microscopy. To acquire biologically relevant data, it is necessary to minimize stress and avoid physical damage to plant tissues during the installation of the sample into the microscope. This is challenging, particularly when the whole plant is mounted as the living sample needs to be properly anchored in the microscopic system to obtain high-quality and high-resolution data. Here, we present a user-optimized sample holder EasyClick for live cell inverted confocal microscopic analysis of plant roots with diameters from 0.3 to 0.7 mm. The EasyClick holder was tested on an inverted confocal microscope using germinating plants of several cereals. Nevertheless, it can be directly used on other types of inverted microscopes from various producers and on different plant species. The EasyClick holder effectively restricts root lateral and vertical movements. This greatly improves the conditions for time-lapse microscopy of the samples of interest.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA21-02929S" target="_blank" >GA21-02929S: Identifikace a charakterizace imprintovaných genů v průběhu vývoje semen ječmene</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Planta

ISSN

0032-0935

e-ISSN

1432-2048

Svazek periodika

259

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

22

Kód UT WoS článku

001125097100001

EID výsledku v databázi Scopus

2-s2.0-85179685820