Anti-inflammatory and antiproliferative activity of Helichrysum odoratissimum Sweet. against lung cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00601085" target="_blank" >RIV/61389030:_____/24:00601085 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.sajb.2024.01.056" target="_blank" >https://doi.org/10.1016/j.sajb.2024.01.056</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sajb.2024.01.056" target="_blank" >10.1016/j.sajb.2024.01.056</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anti-inflammatory and antiproliferative activity of Helichrysum odoratissimum Sweet. against lung cancer

Popis výsledku v původním jazyce

Lung cancer remains the top killing cancer worldwide despite advances in treatment. Seven ethanolic plant extracts were selected and evaluated for their antiproliferative activity against the two main types of lung cancers: non-small cell (A549) and small cell lung cancer cells (SHP-77). An ethanolic extract of Helichrysum odoratissimum Sweet (HO) showed significant antiproliferative activity against lung cancer, with a fifty percent inhibitory concentration (IC50) of 83.43 +/- 1.60 mu g/mL (A549), 49.46 +/- 0.48 mu g/mL (SHP-77) and 50.71 +/- 2.27 mu g/mL, against normal lung epithelial cells (MRC-5), resulting in a selectivity index (SI) value of 0.61 on A549 cells and 1.03 on SHP-77 cells, which was compared to the positive drug control, actinomycin D where the SI values were found to be 2 and 0.25 against A549 and SHP-77 cells, respectively. Against murine macrophages (RAW 264.7) and hepatocytes (HepG2), the HO ethanolic extract showed IC50 values of 60.15 +/- 1.98 mu g/mL and 23.61 +/- 1.06 mu g/mL, respectively. Microscopy showed that the HO ethanolic extract induced apoptosis in the A549 and HepG2 cells at 50 mu g/mL and 300 mu g/mL, respectively. The HO ethanolic extract, furthermore, inhibited the pro-inflammatory enzymes, cyclooxygenase 2 (COX-2) and 5-lipoxygenase (5-LOX) with IC50 values of 7.94 +/- 3.84 mu g/mL and 2.08 +/- 1.35 mu g/mL, respectively, whereas the positive controls Ibuprofen (COX-2) and Zileuton (5-LOX) showed IC50 values of 0.85 +/- 0.14 mu g/mL and 0.06 +/- 0.05 mu g/mL, respectively. The activity of NAD(P)H quinone oxidoreductase-1 (NQO1), which is a direct target of nuclear factor erythroid-2-related factor-2 (NRF2), was significantly inhibited in the A549 cells by the HO ethanolic extract (at 125 mu g/mL) when compared to the positive control, brusatol (at 500 nM). Using the ex ovo yolk sac membrane (YSM) assay, the HO ethanolic extract (at 18.5 mu g/egg) showed a 31.65 +/- 12.80% inhibition of blood vessel formation. This is the first report of the noteworthy antiproliferative activity of the HO ethanolic extract on lung cancer cells including its potential to target several enzymes associated with inflammation and therefore, should be considered for further analysis.

Název v anglickém jazyce

Anti-inflammatory and antiproliferative activity of Helichrysum odoratissimum Sweet. against lung cancer

Popis výsledku anglicky

Lung cancer remains the top killing cancer worldwide despite advances in treatment. Seven ethanolic plant extracts were selected and evaluated for their antiproliferative activity against the two main types of lung cancers: non-small cell (A549) and small cell lung cancer cells (SHP-77). An ethanolic extract of Helichrysum odoratissimum Sweet (HO) showed significant antiproliferative activity against lung cancer, with a fifty percent inhibitory concentration (IC50) of 83.43 +/- 1.60 mu g/mL (A549), 49.46 +/- 0.48 mu g/mL (SHP-77) and 50.71 +/- 2.27 mu g/mL, against normal lung epithelial cells (MRC-5), resulting in a selectivity index (SI) value of 0.61 on A549 cells and 1.03 on SHP-77 cells, which was compared to the positive drug control, actinomycin D where the SI values were found to be 2 and 0.25 against A549 and SHP-77 cells, respectively. Against murine macrophages (RAW 264.7) and hepatocytes (HepG2), the HO ethanolic extract showed IC50 values of 60.15 +/- 1.98 mu g/mL and 23.61 +/- 1.06 mu g/mL, respectively. Microscopy showed that the HO ethanolic extract induced apoptosis in the A549 and HepG2 cells at 50 mu g/mL and 300 mu g/mL, respectively. The HO ethanolic extract, furthermore, inhibited the pro-inflammatory enzymes, cyclooxygenase 2 (COX-2) and 5-lipoxygenase (5-LOX) with IC50 values of 7.94 +/- 3.84 mu g/mL and 2.08 +/- 1.35 mu g/mL, respectively, whereas the positive controls Ibuprofen (COX-2) and Zileuton (5-LOX) showed IC50 values of 0.85 +/- 0.14 mu g/mL and 0.06 +/- 0.05 mu g/mL, respectively. The activity of NAD(P)H quinone oxidoreductase-1 (NQO1), which is a direct target of nuclear factor erythroid-2-related factor-2 (NRF2), was significantly inhibited in the A549 cells by the HO ethanolic extract (at 125 mu g/mL) when compared to the positive control, brusatol (at 500 nM). Using the ex ovo yolk sac membrane (YSM) assay, the HO ethanolic extract (at 18.5 mu g/egg) showed a 31.65 +/- 12.80% inhibition of blood vessel formation. This is the first report of the noteworthy antiproliferative activity of the HO ethanolic extract on lung cancer cells including its potential to target several enzymes associated with inflammation and therefore, should be considered for further analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

South African Journal of Botany

ISSN

0254-6299

e-ISSN

1727-9321

Svazek periodika

166

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

525-538

Kód UT WoS článku

001177291000001

EID výsledku v databázi Scopus

2-s2.0-85184072825