Seed biopriming and long-term air-dry storage effects on Pseudomonas fluorescens viability and Brassica napus germination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00638722" target="_blank" >RIV/61389030:_____/25:00638722 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1017/S0960258525000054" target="_blank" >https://doi.org/10.1017/S0960258525000054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0960258525000054" target="_blank" >10.1017/S0960258525000054</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Seed biopriming and long-term air-dry storage effects on Pseudomonas fluorescens viability and Brassica napus germination

Popis výsledku v původním jazyce

Seed biopriming with Pseudomonas fluorescens as a beneficial microbial inoculant and seed hydropriming with deionized water were conducted with oilseed rape (Brassica napus). Both techniques involve restricted seed hydration followed by seed drying. Seed biopriming reduced the uniformity (time difference between 10 and 90% germination) of germination ca 4-fold, without changing the maximum germination percentages (G(max)) of seed populations. In contrast to this, seed hydropriming improved the uniformity, but not for aged seed populations. The distinct effect of biopriming on germination was caused by the high salt concentration in the priming medium, not by the bacteria or any of the other components. The effects of biopriming duration, seed input and temperature (incubation and drying) were tested and the number of bacteria attached to the seed coat surface was between 1.6 x 10(6) and 9.8 x 10(8) colony-forming units (CFUs) per seed. Long-term storage (21 degrees C, <10% relative humidity, 21% oxygen) of dry bioprimed seeds resulted in a rapid decline of bacterial viability, for example (6 h biopriming, 50 g seed input) from 9.8 x 10(8) CFU per seed to 7.3 x 10(4) after 4 weeks and 5.0 x 10(2) after 12 weeks of air-dry seed storage. Seed biopriming and long-term storage of dry bioprimed seeds did not affect G(max) at optimal (24 degrees C) and cold-stress (16 degrees C) temperatures, and did not appreciably affect early seedling growth. Additive biopriming with kimchi paste did not affect the number of bacteria attached per seed but caused an similar to 800-fold increase in retaining bacterial viability during long-term seed storage.

Název v anglickém jazyce

Seed biopriming and long-term air-dry storage effects on Pseudomonas fluorescens viability and Brassica napus germination

Popis výsledku anglicky

Seed biopriming with Pseudomonas fluorescens as a beneficial microbial inoculant and seed hydropriming with deionized water were conducted with oilseed rape (Brassica napus). Both techniques involve restricted seed hydration followed by seed drying. Seed biopriming reduced the uniformity (time difference between 10 and 90% germination) of germination ca 4-fold, without changing the maximum germination percentages (G(max)) of seed populations. In contrast to this, seed hydropriming improved the uniformity, but not for aged seed populations. The distinct effect of biopriming on germination was caused by the high salt concentration in the priming medium, not by the bacteria or any of the other components. The effects of biopriming duration, seed input and temperature (incubation and drying) were tested and the number of bacteria attached to the seed coat surface was between 1.6 x 10(6) and 9.8 x 10(8) colony-forming units (CFUs) per seed. Long-term storage (21 degrees C, <10% relative humidity, 21% oxygen) of dry bioprimed seeds resulted in a rapid decline of bacterial viability, for example (6 h biopriming, 50 g seed input) from 9.8 x 10(8) CFU per seed to 7.3 x 10(4) after 4 weeks and 5.0 x 10(2) after 12 weeks of air-dry seed storage. Seed biopriming and long-term storage of dry bioprimed seeds did not affect G(max) at optimal (24 degrees C) and cold-stress (16 degrees C) temperatures, and did not appreciably affect early seedling growth. Additive biopriming with kimchi paste did not affect the number of bacteria attached per seed but caused an similar to 800-fold increase in retaining bacterial viability during long-term seed storage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Seed Science Research

ISSN

0960-2585

e-ISSN

1475-2735

Svazek periodika

35

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

18

Strana od-do

60-77

Kód UT WoS článku

001467601300001

EID výsledku v databázi Scopus

2-s2.0-105002687175