Distinct mRNA and miRNA responses in Chlamydomonas reinhardtii reveal particle-specific adaptive mechanisms to ZnO nanoparticles and ZnO bulk

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00639094" target="_blank" >RIV/61389030:_____/25:00639094 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/25:00639094 RIV/62156489:43210/25:43927387 RIV/00216305:26220/26:0200265

Výsledek na webu

<a href="https://doi.org/10.1016/j.algal.2025.104266" target="_blank" >https://doi.org/10.1016/j.algal.2025.104266</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2025.104266" target="_blank" >10.1016/j.algal.2025.104266</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distinct mRNA and miRNA responses in Chlamydomonas reinhardtii reveal particle-specific adaptive mechanisms to ZnO nanoparticles and ZnO bulk

Popis výsledku v původním jazyce

We investigated the adaptive responses of Chlamydomonas reinhardtii to ZnO nanoparticles (ZnO NPs) and ZnO bulk (ZnO Bulk) using integrated physiological, transcriptomic, and miRNA analyses. Cryo-TEM and AAS showed that ZnO NPs accumulated at higher levels and were more widely distributed in cells, whereas ZnO Bulk formed larger intracellular structures. Both materials activated oxidative stress defenses and metal homeostasis, but Bulk uniquely induced strong DNA repair, protein folding, and cell cycle pathways, indicating sustained cellular stress. Small RNA profiling revealed broader miRNA modulation under Bulk exposure, targeting genes linked to chromatin regulation, signaling, and stress adaptation. However, the absence of consistent inverse miRNA-mRNA correlations suggests that miRNAs act mainly as modulators, with transcriptional and proteomic reprogramming driving the primary response. These findings highlight how particle size and aggregation state shape both transcriptional and post-transcriptional regulation in algal stress adaptation, providing new insight into microalgal resilience to engineered ZnO materials.

Název v anglickém jazyce

Distinct mRNA and miRNA responses in Chlamydomonas reinhardtii reveal particle-specific adaptive mechanisms to ZnO nanoparticles and ZnO bulk

Popis výsledku anglicky

We investigated the adaptive responses of Chlamydomonas reinhardtii to ZnO nanoparticles (ZnO NPs) and ZnO bulk (ZnO Bulk) using integrated physiological, transcriptomic, and miRNA analyses. Cryo-TEM and AAS showed that ZnO NPs accumulated at higher levels and were more widely distributed in cells, whereas ZnO Bulk formed larger intracellular structures. Both materials activated oxidative stress defenses and metal homeostasis, but Bulk uniquely induced strong DNA repair, protein folding, and cell cycle pathways, indicating sustained cellular stress. Small RNA profiling revealed broader miRNA modulation under Bulk exposure, targeting genes linked to chromatin regulation, signaling, and stress adaptation. However, the absence of consistent inverse miRNA-mRNA correlations suggests that miRNAs act mainly as modulators, with transcriptional and proteomic reprogramming driving the primary response. These findings highlight how particle size and aggregation state shape both transcriptional and post-transcriptional regulation in algal stress adaptation, providing new insight into microalgal resilience to engineered ZnO materials.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

2211-9264

Svazek periodika

91

Číslo periodika v rámci svazku

OCT

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

104266

Kód UT WoS článku

001566971700001

EID výsledku v databázi Scopus

2-s2.0-105014841149