Ketamine/xylazine anaesthesia in the chronobiological studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F14%3AA1501BT8" target="_blank" >RIV/61988987:17110/14:A1501BT8 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Ketamine/xylazine anaesthesia in the chronobiological studies

Popis výsledku v původním jazyce

Administration of anaesthesia during the daytime is important and may influence specific aspects of in vivo animal experiments. The aim of the present study was to refer to the suitability of the use of ketamine/xylazine anaesthesia in chronobiological studies. Study was performed in ketamine-/xylazine-anaesthetized female Wistar rats ( 100 mg/kg/15 mg/kg, i.m) after adaptation to a light-dark (LD) cycle ( 12 h: 12 h). Heart rate (HR), rectal temperature, ECG parameters, autonomic nervous system activity, acid-base balance and Na+, K+, Ca2+ and Cl(-)plasma concentrations were evaluated in dependence on LD cycle. LD differences were found in the HR, rectal temperature, RR, PQ and QTc intervals, QRS complex, P wave amplitude, in power HF, LF and VLF components, in paO2 and HCO3-, and in Na+, Cl(-)and Ca2+ ions. Ketamine/xylazine anaesthesia may be applicable in chronobiological studies. It does not disturb LD differences, but it can modify them probably without loss of rhythmicity.

Název v anglickém jazyce

Ketamine/xylazine anaesthesia in the chronobiological studies

Popis výsledku anglicky

Administration of anaesthesia during the daytime is important and may influence specific aspects of in vivo animal experiments. The aim of the present study was to refer to the suitability of the use of ketamine/xylazine anaesthesia in chronobiological studies. Study was performed in ketamine-/xylazine-anaesthetized female Wistar rats ( 100 mg/kg/15 mg/kg, i.m) after adaptation to a light-dark (LD) cycle ( 12 h: 12 h). Heart rate (HR), rectal temperature, ECG parameters, autonomic nervous system activity, acid-base balance and Na+, K+, Ca2+ and Cl(-)plasma concentrations were evaluated in dependence on LD cycle. LD differences were found in the HR, rectal temperature, RR, PQ and QTc intervals, QRS complex, P wave amplitude, in power HF, LF and VLF components, in paO2 and HCO3-, and in Na+, Cl(-)and Ca2+ ions. Ketamine/xylazine anaesthesia may be applicable in chronobiological studies. It does not disturb LD differences, but it can modify them probably without loss of rhythmicity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOL RHYTHM RES

ISSN

0929-1016

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

633-642

Kód UT WoS článku

000337580100015

EID výsledku v databázi Scopus

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