Série imunoextrakčních metod na efektivní purifikaci aromatických cytokininů.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F05%3A00001951" target="_blank" >RIV/61989592:15110/05:00001951 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Batch immunoextraction method for an effective purification of aromatic cytokinins.

Popis výsledku v původním jazyce

Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under invitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurinesand the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand

Název v anglickém jazyce

Batch immunoextraction method for an effective purification of aromatic cytokinins.

Popis výsledku anglicky

Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under invitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurinesand the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A: Symposium Volumes

ISSN

0021-9673

e-ISSN

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Svazek periodika

1100

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

116-125

Kód UT WoS článku

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EID výsledku v databázi Scopus

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