Polyphenolic fraction of Lonicera caerulea L. fruits reduces oxidative stress and inflammatory markers induced by lipopolysaccharide in gingival fibroblasts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10213260" target="_blank" >RIV/61989592:15110/10:10213260 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polyphenolic fraction of Lonicera caerulea L. fruits reduces oxidative stress and inflammatory markers induced by lipopolysaccharide in gingival fibroblasts

Popis výsledku v původním jazyce

The most common oral diseases have a microbial aetiology. Pathogenic bacteria liberate a number of irritating agents including a lipopolysaccharide (LPS) that activates pro-inflammatory cytokines promoting increased activity of polymorphonucleocytes (PMN). Berries of Lonicera caerulea L. are rich in phenolic acids, flavonoids and anthocyanins that have multiple biological activities in vitro and in vivo. Studies have shown that polyphenols suppress a number of LPS-induced signals. We assessed effects ofthe polyphenolic fraction of L. caerulea fruits (PFLC; 77% anthocyanins) on LPS-induced oxidative damage and inflammation in human gingival fibroblasts. PFLC (10-50mug/ml) reduced reactive oxygen species production, intracellular glutathione depletion and lipid peroxidation in LPS-treated cells. PFLC treatment also inhibited LPS-induced up-regulation of interleukin-1beta, interleukin-6 and tumour necrosis factor-alpha and it suppressed expression of cyclooxygenase-2.

Název v anglickém jazyce

Polyphenolic fraction of Lonicera caerulea L. fruits reduces oxidative stress and inflammatory markers induced by lipopolysaccharide in gingival fibroblasts

Popis výsledku anglicky

The most common oral diseases have a microbial aetiology. Pathogenic bacteria liberate a number of irritating agents including a lipopolysaccharide (LPS) that activates pro-inflammatory cytokines promoting increased activity of polymorphonucleocytes (PMN). Berries of Lonicera caerulea L. are rich in phenolic acids, flavonoids and anthocyanins that have multiple biological activities in vitro and in vivo. Studies have shown that polyphenols suppress a number of LPS-induced signals. We assessed effects ofthe polyphenolic fraction of L. caerulea fruits (PFLC; 77% anthocyanins) on LPS-induced oxidative damage and inflammation in human gingival fibroblasts. PFLC (10-50mug/ml) reduced reactive oxygen species production, intracellular glutathione depletion and lipid peroxidation in LPS-treated cells. PFLC treatment also inhibited LPS-induced up-regulation of interleukin-1beta, interleukin-6 and tumour necrosis factor-alpha and it suppressed expression of cyclooxygenase-2.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/FT-TA3%2F024" target="_blank" >FT-TA3/024: Rostlinná chemoprotektiva - Nové doplňky stravy pro zdraví a prevenci před chronickými nemocemi.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and Chemical Toxicology

ISSN

0278-6915

e-ISSN

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Svazek periodika

48

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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