Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours -- IPO8 and RPL4 are reliable reference genes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33144170" target="_blank" >RIV/61989592:15110/13:33144170 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1757-2215-6-60" target="_blank" >http://dx.doi.org/10.1186/1757-2215-6-60</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1757-2215-6-60" target="_blank" >10.1186/1757-2215-6-60</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours -- IPO8 and RPL4 are reliable reference genes

Popis výsledku v původním jazyce

To ensure a correct interpretation of results obtained with quantitative real-time reverse transcriptionpolymerase chain reaction (RT-qPCR), it is critical to normalize to a reference gene with stable mRNA expression in the tissue of interest. GADPH is widely used as a reference gene in ovarian tumour studies, although lacking tissue-specific stability. The aim of this study was to identify alternative suitable reference genes for RT-qPCR studies on benign, borderline, and malignant ovarian tumours. Weassayed mRNA levels for 13 potential reference genes ? ABL1, ACTB, CDKN1A, GADPH, GUSB, HPRT1, HSP90AB, IPO8, PPIA, RPL30, RPL4, RPLPO, and TBP ?with RT-qPCR in 42 primary ovarian tumours, using commercially pre-designed RT-qPCR probes. Expression stability was subsequently analysed with four different statistical programs (GeNorm, NormFinder, BestKeeper, and the Equivalence test). Expression of IPO8, RPL4, TBP, RPLPO, and ACTB had the least variation in expression across the tumour samp

Název v anglickém jazyce

Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours -- IPO8 and RPL4 are reliable reference genes

Popis výsledku anglicky

To ensure a correct interpretation of results obtained with quantitative real-time reverse transcriptionpolymerase chain reaction (RT-qPCR), it is critical to normalize to a reference gene with stable mRNA expression in the tissue of interest. GADPH is widely used as a reference gene in ovarian tumour studies, although lacking tissue-specific stability. The aim of this study was to identify alternative suitable reference genes for RT-qPCR studies on benign, borderline, and malignant ovarian tumours. Weassayed mRNA levels for 13 potential reference genes ? ABL1, ACTB, CDKN1A, GADPH, GUSB, HPRT1, HSP90AB, IPO8, PPIA, RPL30, RPL4, RPLPO, and TBP ?with RT-qPCR in 42 primary ovarian tumours, using commercially pre-designed RT-qPCR probes. Expression stability was subsequently analysed with four different statistical programs (GeNorm, NormFinder, BestKeeper, and the Equivalence test). Expression of IPO8, RPL4, TBP, RPLPO, and ACTB had the least variation in expression across the tumour samp

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Jornal of Ovarian Research

ISSN

1757-2215

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

60-69

Kód UT WoS článku

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EID výsledku v databázi Scopus

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