Cytokinin-induced cell death is associated with elevated expression of alternative oxidase in tobacco BY-2 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33146084" target="_blank" >RIV/61989592:15110/13:33146084 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00709-013-0501-3" target="_blank" >http://dx.doi.org/10.1007/s00709-013-0501-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00709-013-0501-3" target="_blank" >10.1007/s00709-013-0501-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinin-induced cell death is associated with elevated expression of alternative oxidase in tobacco BY-2 cells

Popis výsledku v původním jazyce

N6-benzyladenine (BA) and N6-benzyladenosine ([9R]BA) induce massive production of ROS that is eventually followed by a loss of cell viability in tobacco BY-2 cells (Mlejnek et al. 2003; Mlejnek et al. 2005). Results presented in this work suggest that the main sources of ROS are likely mitochondria and that the maintenance of the mitochondrial transmembrane potential is crucial for ROS production in cytokinin treaded BY-2 cells. Therefore, the possible involvement of alternative oxidase (AOX) in cell death process induced by BA and [9R]BA was studied. About three to four fold increase in mRNA levels of AOX1 was observed a few hours after the BA and [9R]BA addition into the growth medium. The elevated expression of AOX1 mRNA could be prevented by adding adenine and adenosine which simultaneously reduced the cytotoxic effects of BA and [9R]BA, respectively. N6-benzyladenine 7-ß-D-glucoside ([7G]BA) which is a common non-toxic metabolite of BA and [9R]BA did not affect the AOX1 mRNA expr

Název v anglickém jazyce

Cytokinin-induced cell death is associated with elevated expression of alternative oxidase in tobacco BY-2 cells

Popis výsledku anglicky

N6-benzyladenine (BA) and N6-benzyladenosine ([9R]BA) induce massive production of ROS that is eventually followed by a loss of cell viability in tobacco BY-2 cells (Mlejnek et al. 2003; Mlejnek et al. 2005). Results presented in this work suggest that the main sources of ROS are likely mitochondria and that the maintenance of the mitochondrial transmembrane potential is crucial for ROS production in cytokinin treaded BY-2 cells. Therefore, the possible involvement of alternative oxidase (AOX) in cell death process induced by BA and [9R]BA was studied. About three to four fold increase in mRNA levels of AOX1 was observed a few hours after the BA and [9R]BA addition into the growth medium. The elevated expression of AOX1 mRNA could be prevented by adding adenine and adenosine which simultaneously reduced the cytotoxic effects of BA and [9R]BA, respectively. N6-benzyladenine 7-ß-D-glucoside ([7G]BA) which is a common non-toxic metabolite of BA and [9R]BA did not affect the AOX1 mRNA expr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Protoplasma: an international journal of cell biology

ISSN

0033-183X

e-ISSN

—

Svazek periodika

250

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

1195-1202

Kód UT WoS článku

000325132800021

EID výsledku v databázi Scopus

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