Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F14%3A33148768" target="_blank" >RIV/61989592:15110/14:33148768 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61389005:_____/14:00427322
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.fsigen.2013.10.009" target="_blank" >http://dx.doi.org/10.1016/j.fsigen.2013.10.009</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fsigen.2013.10.009" target="_blank" >10.1016/j.fsigen.2013.10.009</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool
Popis výsledku v původním jazyce
The capability of Fluorescent Random Amplified Microsatellites (F-RAMS) to profile hallucinogenic mushrooms to species and sub-species level was assessed. Fifteen samples of Amanita rubescens and 22 samples of other hallucinogenic and non-hallucinogenicmushrooms of the genera Amanita and Psilocybe were profiled using two fluorescently-labeled, 50degenerate primers, 50-6FAM-SpC3-DD (CCA)5 and 50- 6FAM-SpC3-DHB (CGA)5, which target different microsatellite repeat regions. Among the two primers, 50-6FAM-SpC3-DHB (CGA)5 provided more reliable data for identification purposes, by grouping samples of the same species and clustering closely related species together in a dendrogram based on amplicon similarities. A high degree of intra-specific variation between the 15 A. rubescens samples was shown with both primers and the amplicons generated for all A. rubescens samples were organized into three classes of amplicons (discriminant, private, and marker) based on their individualizing potenti
Název v anglickém jazyce
Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool
Popis výsledku anglicky
The capability of Fluorescent Random Amplified Microsatellites (F-RAMS) to profile hallucinogenic mushrooms to species and sub-species level was assessed. Fifteen samples of Amanita rubescens and 22 samples of other hallucinogenic and non-hallucinogenicmushrooms of the genera Amanita and Psilocybe were profiled using two fluorescently-labeled, 50degenerate primers, 50-6FAM-SpC3-DD (CCA)5 and 50- 6FAM-SpC3-DHB (CGA)5, which target different microsatellite repeat regions. Among the two primers, 50-6FAM-SpC3-DHB (CGA)5 provided more reliable data for identification purposes, by grouping samples of the same species and clustering closely related species together in a dendrogram based on amplicon similarities. A high degree of intra-specific variation between the 15 A. rubescens samples was shown with both primers and the amplicons generated for all A. rubescens samples were organized into three classes of amplicons (discriminant, private, and marker) based on their individualizing potenti
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicína pro regionální rozvoj a lidské zdroje</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Forensic Science International-Genetics
ISSN
1872-4973
e-ISSN
—
Svazek periodika
9
Číslo periodika v rámci svazku
duben 2014
Stát vydavatele periodika
IE - Irsko
Počet stran výsledku
8
Strana od-do
25-32
Kód UT WoS článku
000331834600019
EID výsledku v databázi Scopus
—