Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F14%3A33148768" target="_blank" >RIV/61989592:15110/14:33148768 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/14:00427322

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fsigen.2013.10.009" target="_blank" >http://dx.doi.org/10.1016/j.fsigen.2013.10.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fsigen.2013.10.009" target="_blank" >10.1016/j.fsigen.2013.10.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool

Popis výsledku v původním jazyce

The capability of Fluorescent Random Amplified Microsatellites (F-RAMS) to profile hallucinogenic mushrooms to species and sub-species level was assessed. Fifteen samples of Amanita rubescens and 22 samples of other hallucinogenic and non-hallucinogenicmushrooms of the genera Amanita and Psilocybe were profiled using two fluorescently-labeled, 50degenerate primers, 50-6FAM-SpC3-DD (CCA)5 and 50- 6FAM-SpC3-DHB (CGA)5, which target different microsatellite repeat regions. Among the two primers, 50-6FAM-SpC3-DHB (CGA)5 provided more reliable data for identification purposes, by grouping samples of the same species and clustering closely related species together in a dendrogram based on amplicon similarities. A high degree of intra-specific variation between the 15 A. rubescens samples was shown with both primers and the amplicons generated for all A. rubescens samples were organized into three classes of amplicons (discriminant, private, and marker) based on their individualizing potenti

Název v anglickém jazyce

Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool

Popis výsledku anglicky

The capability of Fluorescent Random Amplified Microsatellites (F-RAMS) to profile hallucinogenic mushrooms to species and sub-species level was assessed. Fifteen samples of Amanita rubescens and 22 samples of other hallucinogenic and non-hallucinogenicmushrooms of the genera Amanita and Psilocybe were profiled using two fluorescently-labeled, 50degenerate primers, 50-6FAM-SpC3-DD (CCA)5 and 50- 6FAM-SpC3-DHB (CGA)5, which target different microsatellite repeat regions. Among the two primers, 50-6FAM-SpC3-DHB (CGA)5 provided more reliable data for identification purposes, by grouping samples of the same species and clustering closely related species together in a dendrogram based on amplicon similarities. A high degree of intra-specific variation between the 15 A. rubescens samples was shown with both primers and the amplicons generated for all A. rubescens samples were organized into three classes of amplicons (discriminant, private, and marker) based on their individualizing potenti

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicína pro regionální rozvoj a lidské zdroje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forensic Science International-Genetics

ISSN

1872-4973

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

duben 2014

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

8

Strana od-do

25-32

Kód UT WoS článku

000331834600019

EID výsledku v databázi Scopus

—