Caracasine, An ent-kaurane Diterpene with Proapoptotic and Pro-differentiator Activity in Human Leukaemia Cell Lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F23%3A73619715" target="_blank" >RIV/61989592:15110/23:73619715 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15640/23:73619715

Výsledek na webu

<a href="https://www.eurekaselect.com/article/122591" target="_blank" >https://www.eurekaselect.com/article/122591</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2174/1871520622666220415105615" target="_blank" >10.2174/1871520622666220415105615</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Caracasine, An ent-kaurane Diterpene with Proapoptotic and Pro-differentiator Activity in Human Leukaemia Cell Lines

Popis výsledku v původním jazyce

Background: Kaurane-type diterpenoids, obtained from various natural sources, have shown many biological activities, including anti-inflammatory and antitumor effects. Caracasine, an ent-kaurane diterpenoid isolated from the flowers of Croton micans, was shown to induce apoptosis in leukaemia cell lines. Objective: The present study aimed to ascertain the compound’s mechanism of cell death induction using two leukaemia cell lines, Jurkat E6.1 (T cell) and HL-60 (promyeloblast cells). Methods: Cell death in Jurkat and HL60 cells were evaluated by flow cytometry for apoptosis with annexin-V/PI, mitochondrial membrane potential disturbance, changes in cell cycle, CD95 expression, caspase activation, Nuclear Factor kappa B inhibition, and differentiation into a neutrophil-like cell (dHL60). Results: Caracasine (10 µM) increased the G0/G1 phase in Jurkat and arrested the cell cycle in the S phase in HL60. Caracasine increased CD95 expression (p&lt;0.01 in Jurkat and p&lt;0.05 in HL60) and caspase-8 activation (p&lt;0.001 in Jurkat and p&lt;0.05 in HL60). Caspase-9 was activated in both cell lines (p&lt;0.001) along with the decline in mitochon-drial ΔΨm (p&lt;0.05 in Jurkat and p[removed]

Název v anglickém jazyce

Caracasine, An ent-kaurane Diterpene with Proapoptotic and Pro-differentiator Activity in Human Leukaemia Cell Lines

Popis výsledku anglicky

Background: Kaurane-type diterpenoids, obtained from various natural sources, have shown many biological activities, including anti-inflammatory and antitumor effects. Caracasine, an ent-kaurane diterpenoid isolated from the flowers of Croton micans, was shown to induce apoptosis in leukaemia cell lines. Objective: The present study aimed to ascertain the compound’s mechanism of cell death induction using two leukaemia cell lines, Jurkat E6.1 (T cell) and HL-60 (promyeloblast cells). Methods: Cell death in Jurkat and HL60 cells were evaluated by flow cytometry for apoptosis with annexin-V/PI, mitochondrial membrane potential disturbance, changes in cell cycle, CD95 expression, caspase activation, Nuclear Factor kappa B inhibition, and differentiation into a neutrophil-like cell (dHL60). Results: Caracasine (10 µM) increased the G0/G1 phase in Jurkat and arrested the cell cycle in the S phase in HL60. Caracasine increased CD95 expression (p&lt;0.01 in Jurkat and p&lt;0.05 in HL60) and caspase-8 activation (p&lt;0.001 in Jurkat and p&lt;0.05 in HL60). Caspase-9 was activated in both cell lines (p&lt;0.001) along with the decline in mitochon-drial ΔΨm (p&lt;0.05 in Jurkat and p[removed]

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anti-Cancer Agents in Medicinal Chemistry

ISSN

1871-5206

e-ISSN

1875-5992

Svazek periodika

23

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

AE - Spojené arabské emiráty

Počet stran výsledku

11

Strana od-do

1145-1155

Kód UT WoS článku

001025442300004

EID výsledku v databázi Scopus

2-s2.0-85161025667