The development of biosensor for the determination of amylase activity

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The development of biosensor for the determination of amylase activity

Popis výsledku v původním jazyce

a-Amylase (EC 3.2.1.1) catalyze the hydrolysis of starch to a significant extent resulting in the release of maltose. Determination of serum a-amylase activity is a procedure widely used, for example, in clinical medicine for the diagnosis of acute pancreatitis. The procedures most frequently used to determine a-amylase activity are either amyloclastic or saccharogenic (MAZZUCHIN et al., 1973). The former is based on the starch-iodine reaction and the latter on the formation of reducing sugars, measuredas maltose or glucose equivalents. Biosensor determination of maltose is based on an oxygen Clark type electrode equipped with a suitable enzymatic membrane (FILIPIAK et al.,1996). This membrane can be obtained by immobilization of the enzymes hydrolysing maltose (a-glucosidase) to glucose, which is determined using an oxygen electrode with the glucose oxidase membrane. The new method of determination of ?-amylase activity is based on determination of produced maltose using the Clark el

Název v anglickém jazyce

The development of biosensor for the determination of amylase activity

Popis výsledku anglicky

a-Amylase (EC 3.2.1.1) catalyze the hydrolysis of starch to a significant extent resulting in the release of maltose. Determination of serum a-amylase activity is a procedure widely used, for example, in clinical medicine for the diagnosis of acute pancreatitis. The procedures most frequently used to determine a-amylase activity are either amyloclastic or saccharogenic (MAZZUCHIN et al., 1973). The former is based on the starch-iodine reaction and the latter on the formation of reducing sugars, measuredas maltose or glucose equivalents. Biosensor determination of maltose is based on an oxygen Clark type electrode equipped with a suitable enzymatic membrane (FILIPIAK et al.,1996). This membrane can be obtained by immobilization of the enzymes hydrolysing maltose (a-glucosidase) to glucose, which is determined using an oxygen electrode with the glucose oxidase membrane. The new method of determination of ?-amylase activity is based on determination of produced maltose using the Clark el

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

18th Joint Congress of the Czech and Slovak Societies for Biochemistry and Molecular Biology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

410

Strana od-do

148

Název nakladatele

Slovenská spoločnosť pre biochémiu a molekulárnu biológiu

Místo vydání

Bratislava

Místo konání akce

Stará Lesná

Datum konání akce

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Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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