Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F10%3A10215687" target="_blank" >RIV/61989592:15310/10:10215687 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry

Popis výsledku v původním jazyce

A fast and precise analysis of the synthetic peptide buserelin in urine using CZE-ESI-MS method has been demonstrated. Formic acid at 50 mmol/L concentration served as backgroud electrolyte in CZE stage and it is compatible with MS detection in positiveionization mode. Two injection modes were tested, i.e. pressure (50 mbar for 5 s) and electrokinetic injection (5 kV for 5 s), of which electrokinetic injection provided better calibration parameters. Buserelin LODs were 0 47 mu g/mL in water and 0 63 mug/mL in ten times diluted urine samples using pressure injection, while they were 0.32 mu g/mL in water and 0.34 mu g/mL in ten times diluted urine samples using electrokinetic injection Repeatability of buserelin migration times was below 6% (pressureinjection mode) and 1% (electrokinetic injection mode).

Název v anglickém jazyce

Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry

Popis výsledku anglicky

A fast and precise analysis of the synthetic peptide buserelin in urine using CZE-ESI-MS method has been demonstrated. Formic acid at 50 mmol/L concentration served as backgroud electrolyte in CZE stage and it is compatible with MS detection in positiveionization mode. Two injection modes were tested, i.e. pressure (50 mbar for 5 s) and electrokinetic injection (5 kV for 5 s), of which electrokinetic injection provided better calibration parameters. Buserelin LODs were 0 47 mu g/mL in water and 0 63 mug/mL in ten times diluted urine samples using pressure injection, while they were 0.32 mu g/mL in water and 0.34 mu g/mL in ten times diluted urine samples using electrokinetic injection Repeatability of buserelin migration times was below 6% (pressureinjection mode) and 1% (electrokinetic injection mode).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

31

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000276811000015

EID výsledku v databázi Scopus

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