High Performance Liquid Chromatography-Electrochemistry-Electrospray Ionization Mass Spectrometry (HPLC/EC/ESI-MS) for Detection and Characterization of Roscovitine Oxidation Products
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F11%3A33118145" target="_blank" >RIV/61989592:15310/11:33118145 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61389030:_____/11:00374545 RIV/70883521:28610/12:43866427
Výsledek na webu
<a href="http://dx.doi.org/10.1002/elan.201100383" target="_blank" >http://dx.doi.org/10.1002/elan.201100383</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.201100383" target="_blank" >10.1002/elan.201100383</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
High Performance Liquid Chromatography-Electrochemistry-Electrospray Ionization Mass Spectrometry (HPLC/EC/ESI-MS) for Detection and Characterization of Roscovitine Oxidation Products
Popis výsledku v původním jazyce
On-line LC-EC/ESI-MS has been established as a fast and simple method to mimic some types of oxidation reaction of various drugs and to study the formation and structure of the resulting products. This technique has been applied to a 2,6,9-trisubstitutedpurine, R-roscovitine, which is known to be an inhibitor of some cyclin-dependent kinases (CDKs) and a potential anticancer drug. Oxidation of R-roscovitine in an electrochemical cell (EC), operated under various conditions, resulted in appearance of 6major products. These were further analyzed by high-resolution mass spectrometry, their structures were elucidated by accurate mass measurement and compared to previously identified R-roscovitine in vitro/in vivo metabolites. Although none of the observed products was structurally identical to those identified previously in vitro/in vivo, all of them, except for the methoxylated products, resembled similarity due to appearing through the same reaction type. R-roscovitine in the EC cell u
Název v anglickém jazyce
High Performance Liquid Chromatography-Electrochemistry-Electrospray Ionization Mass Spectrometry (HPLC/EC/ESI-MS) for Detection and Characterization of Roscovitine Oxidation Products
Popis výsledku anglicky
On-line LC-EC/ESI-MS has been established as a fast and simple method to mimic some types of oxidation reaction of various drugs and to study the formation and structure of the resulting products. This technique has been applied to a 2,6,9-trisubstitutedpurine, R-roscovitine, which is known to be an inhibitor of some cyclin-dependent kinases (CDKs) and a potential anticancer drug. Oxidation of R-roscovitine in an electrochemical cell (EC), operated under various conditions, resulted in appearance of 6major products. These were further analyzed by high-resolution mass spectrometry, their structures were elucidated by accurate mass measurement and compared to previously identified R-roscovitine in vitro/in vivo metabolites. Although none of the observed products was structurally identical to those identified previously in vitro/in vivo, all of them, except for the methoxylated products, resembled similarity due to appearing through the same reaction type. R-roscovitine in the EC cell u
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electroanalysis
ISSN
1040-0397
e-ISSN
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Svazek periodika
23
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
8
Strana od-do
2898-2905
Kód UT WoS článku
000298063500018
EID výsledku v databázi Scopus
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