Determination of selected biogenic amines in red wines by automated on-line combination of capillary isotachophoresis-capillary zone electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142148" target="_blank" >RIV/61989592:15310/12:33142148 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jchromb.2012.07.018" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2012.07.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2012.07.018" target="_blank" >10.1016/j.jchromb.2012.07.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of selected biogenic amines in red wines by automated on-line combination of capillary isotachophoresis-capillary zone electrophoresis

Popis výsledku v původním jazyce

A screening analytical method based on an automated on-line combination of capillary isotachophoresis-capillary zone electrophoresis (cITP-CZE) in hydrodynamically closed separation system, equipped with photometric detection at 280 nm. was developed fora routine determination of the selected biogenic amines, namely histamine, 2-phenylethylamine and tyramine, in red wines. The evaluated limits of detection (LODs) were 0.35 mg L-1 for histamine, 0.33 mg L-1 for 2-phenylethylamine and 0.37 mg L-1 for tyramine. The repeatability of the migration time and peak area for histamine were 1.1% and 2.6%, respectively, for 2-phenylethylamine 0.7% and 2.0%, respectively, and for tyramine 0.8% and 2.1%. respectively. The method recoveries were 92.1% for histamine,96.4% for 2-phenylethylamine and 95.5% for tyramine. The developed automated cITP-CZE-UV method was applied for the determination of histamine, 2-phenylethylamine and tyramine in seven red wine samples originating from Czech Republic.

Název v anglickém jazyce

Determination of selected biogenic amines in red wines by automated on-line combination of capillary isotachophoresis-capillary zone electrophoresis

Popis výsledku anglicky

A screening analytical method based on an automated on-line combination of capillary isotachophoresis-capillary zone electrophoresis (cITP-CZE) in hydrodynamically closed separation system, equipped with photometric detection at 280 nm. was developed fora routine determination of the selected biogenic amines, namely histamine, 2-phenylethylamine and tyramine, in red wines. The evaluated limits of detection (LODs) were 0.35 mg L-1 for histamine, 0.33 mg L-1 for 2-phenylethylamine and 0.37 mg L-1 for tyramine. The repeatability of the migration time and peak area for histamine were 1.1% and 2.6%, respectively, for 2-phenylethylamine 0.7% and 2.0%, respectively, and for tyramine 0.8% and 2.1%. respectively. The method recoveries were 92.1% for histamine,96.4% for 2-phenylethylamine and 95.5% for tyramine. The developed automated cITP-CZE-UV method was applied for the determination of histamine, 2-phenylethylamine and tyramine in seven red wine samples originating from Czech Republic.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0058" target="_blank" >ED2.1.00/03.0058: Regionální centrum pokročilých technologií a materiálů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

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Svazek periodika

904

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

135-139

Kód UT WoS článku

000308734400018

EID výsledku v databázi Scopus

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