Rapid UPLC-ESI-MS/MS method for the analysis of isoflavonoids and other phenylpropanoids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142872" target="_blank" >RIV/61989592:15310/12:33142872 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/12:00382519 RIV/60461373:22330/12:43894072

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0889157512000117" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0889157512000117</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2011.12.001" target="_blank" >10.1016/j.jfca.2011.12.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid UPLC-ESI-MS/MS method for the analysis of isoflavonoids and other phenylpropanoids

Popis výsledku v původním jazyce

UPLC-ESI-MS/MS method was developed for the quantitative and qualitative analysis of 26 phenolic compounds in plant material, including 15 isoflavonoids (daidzein, genistein, isoformononetin, formononetin, prunetin, biochanin A. daidzin, genistin, ononin, sissotrin, osajin, pomiferin), 5 flavones (apigenin, kaempferol, quercetin, quercetin-3-glucoside, rutin), 4 flavanones (hesperetin, naringenin, naringenin-7-glucoside, naringin), a coumestan (coumestrol) and a coumarine (scopoletin). The analytes weredetected in a multiple reaction monitoring mode and quantified using internal standards [C-13(3)]daidzin, [H-2(6)]naringenin and [H-2(4)]genistein. Limits of detection were in the range 0.001-10 pmol injection(-1). The precision of the method (relativestandard deviation) ranged from 2.6 to 15.1% and the accuracy was -22.1 to 16.6%. Application of the method was demonstrated by the analysis of mung bean (Vigna radiata) sprout extract, in which 14 of the 26 analytes were detected and qua

Název v anglickém jazyce

Rapid UPLC-ESI-MS/MS method for the analysis of isoflavonoids and other phenylpropanoids

Popis výsledku anglicky

UPLC-ESI-MS/MS method was developed for the quantitative and qualitative analysis of 26 phenolic compounds in plant material, including 15 isoflavonoids (daidzein, genistein, isoformononetin, formononetin, prunetin, biochanin A. daidzin, genistin, ononin, sissotrin, osajin, pomiferin), 5 flavones (apigenin, kaempferol, quercetin, quercetin-3-glucoside, rutin), 4 flavanones (hesperetin, naringenin, naringenin-7-glucoside, naringin), a coumestan (coumestrol) and a coumarine (scopoletin). The analytes weredetected in a multiple reaction monitoring mode and quantified using internal standards [C-13(3)]daidzin, [H-2(6)]naringenin and [H-2(4)]genistein. Limits of detection were in the range 0.001-10 pmol injection(-1). The precision of the method (relativestandard deviation) ranged from 2.6 to 15.1% and the accuracy was -22.1 to 16.6%. Application of the method was demonstrated by the analysis of mung bean (Vigna radiata) sprout extract, in which 14 of the 26 analytes were detected and qua

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

—

Svazek periodika

26

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

36-42

Kód UT WoS článku

000306045700004

EID výsledku v databázi Scopus

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