Bulk derivatization and direct injection of human cerebrospinal fluid for trace-level quantification of endogenous estrogens using trap-and-elute liquid chromatography with tandem mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151656" target="_blank" >RIV/61989592:15310/14:33151656 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400165/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400165/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201400165" target="_blank" >10.1002/jssc.201400165</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bulk derivatization and direct injection of human cerebrospinal fluid for trace-level quantification of endogenous estrogens using trap-and-elute liquid chromatography with tandem mass spectrometry

Popis výsledku v původním jazyce

Although there are existing methods for determining estrogen in human bodily fluids including blood plasma and serum, very little information is available regarding estrogen levels in human cerebrospinal fluid (CSF), which is critical to assess in studies of neuroprotective functions and diffusion of neuroprotective estrogens across the blood-brain barrier. To address this problem, a liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of four endogenous estrogens (estrone, 17 alpha-estradiol, 17 alpha-estradiol, and estriol) in human CSF was developed. An aliquot (300 mu L) of human CSF was bulk derivatized using dansyl chloride in the sample and 10 mu L was directly injected onto a restricted-access media trap column for protein removal. No off-line sample extraction or cleanup was needed. The limits of detection of estrone, 17 alpha-estradiol, 17 beta-estradiol, and estriol were 17, 28, 13, and 30 pg/mL, respectively, which is in the parts-p

Název v anglickém jazyce

Bulk derivatization and direct injection of human cerebrospinal fluid for trace-level quantification of endogenous estrogens using trap-and-elute liquid chromatography with tandem mass spectrometry

Popis výsledku anglicky

Although there are existing methods for determining estrogen in human bodily fluids including blood plasma and serum, very little information is available regarding estrogen levels in human cerebrospinal fluid (CSF), which is critical to assess in studies of neuroprotective functions and diffusion of neuroprotective estrogens across the blood-brain barrier. To address this problem, a liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of four endogenous estrogens (estrone, 17 alpha-estradiol, 17 alpha-estradiol, and estriol) in human CSF was developed. An aliquot (300 mu L) of human CSF was bulk derivatized using dansyl chloride in the sample and 10 mu L was directly injected onto a restricted-access media trap column for protein removal. No off-line sample extraction or cleanup was needed. The limits of detection of estrone, 17 alpha-estradiol, 17 beta-estradiol, and estriol were 17, 28, 13, and 30 pg/mL, respectively, which is in the parts-p

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

2010-2017

Kód UT WoS článku

000339670600015

EID výsledku v databázi Scopus

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